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D5042

Sigma-Aldrich

123 bp DNA Ladder

for DNA electrophoresis

Synonyme(s) :

DNA Ladder for DNA electrophoresis, DNA marker

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About This Item

Code UNSPSC :
41105335
Nomenclature NACRES :
NA.25

Niveau de qualité

200

Forme

liquid

Utilisation

100 uses (0.1 mg size)
250 uses (250 μg size)

Adéquation

suitable for electrophoresis (DNA)

Température de stockage

−20°C

Application

123 bp DNA Ladder has been used as a size marker in random amplified polymorphic DNA-polymerase chain reaction (PCR) analysis. It has also been used as a molecular marker in gel electrophoresis.

Composants

Sigma′s 123 bp DNA Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, and 0.1 mM EDTA.

Autres remarques

Contains 35 fragments, 123 - 4182 bp
For optimal resolution, the recommended agarose gel concentration is 1.5%.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

J L Hartley et al.
Gene, 13(4), 347-353 (1981-05-01)
A method for self-ligation of DNA segments, which is based on the rotational non-equivalence of ends produced by AvaI cleavage, has been developed. Using this method and an initiator molecule to encourage the formation of long polymers, we have obtained
Gene splicing and mutagenesis by PCR-driven overlap extension
Heckman KL and Pease LR
Nature Protocols, 2(4), 924-932 (2007)
E J Gubbins et al.
The Journal of biological chemistry, 255(18), 8655-8662 (1980-09-25)
The organization and sequence of the rat preprolactin gene has been investigated. Analysis of two different plasmids containing pituitary cDNA inserts has provided the complete 681-nucleotide coding sequence of preprolactin as well as 17 nucleotides preceding the initiation codon and
Sabrina Bochicchio et al.
International journal of pharmaceutics, 525(2), 377-387 (2017-02-13)
Tailored developed nanoliposomes loaded with a siRNA against the transcription factor E2F1 (siE2F1), were produced and delivered to human colorectal adenocarcinoma cell lines and to intestinal human biopsies. siE2F1 loaded nanoliposomes were produced through a dedicated ultrasound assisted technique producing

Articles

Markers and Ladders for Gel Electrophoresis

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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