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D3812

Sigma-Aldrich

DirectLoad™ 50 bp DNA Step Ladder

ready-to-use marker for DNA electrophoresis

Synonyme(s) :

DirectLoad™ 50 bp DNA Marker, Ready-to-use 50 bp DNA Marker, Ready-to-use DirectLoad™ 50 bp DNA Marker, Ready-to-use DirectLoad™ 50 bp DNA Step Ladder

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About This Item

Code UNSPSC :
41105335
Nomenclature NACRES :
NA.25

Niveau de qualité

200

Forme

(liquid)

Utilisation

sufficient for 100 loads

Adéquation

suitable for electrophoresis (DNA)

Température de stockage

−20°C

Description générale

Sigma′s DirectLoad™ 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Application

DirectLoad™ 50 bp DNA Step Ladder has been used for cell lysis and genomic cleavage detection assay.
Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Caractéristiques et avantages

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Composants

Sigma′s DirectLoad™ 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol.

Autres remarques

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Informations légales

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Solid-phase reverse transfection for intracellular delivery of functionally active proteins
Bulkescher R, et al.
Genome Research, 27(10), 1752-1758 (2017)
Cinzia Fabbro et al.
FEMS microbiology letters, 307(2), 158-164 (2010-04-24)
Culturable vibrios were isolated from seawater collected during an annual sampling study performed along the Gulf of Trieste coast (Northern Adriatic Sea), and conventional culturing and identification methods were used to investigate the presence of Vibrio parahaemolyticus. Biochemically selected Vibrio

Articles

Markers and Ladders for Gel Electrophoresis

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

Protocoles

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Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Antibody-Enzyme Mediated Hot Start PCR Protocol

When using hot start Taq DNA polymerase, the enzyme remains inactive until heated. Hot Start DNA polymerase control is achieved by chemical or antibody modification of the enzyme.

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