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LUC1

Sigma-Aldrich

Luciferase Reporter Gene Detection Kit

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.84

Utilisation

 kit sufficient for 100 assays

Niveau de qualité

200

Température de stockage

−70°C

Description générale

Firefly luciferase is one of the most utilized reporter genes for the study of gene expression. It is an extremely sensitive, rapid, and easy-to-use reporter gene. The chemiluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. The luciferase substrate contains coenzyme A for increased and sustained luminescence compared to conventional methods. This eliminates the need for automated luminometer injection of the substrate and allows analysis by photographic film or scintillation counting. The lysis buffer contains polymyxin B which further enhances the signal and eliminates lysozyme treatment and freeze-thawing of cells. Cell lysis buffer is compatible with β-galactosidase assays. The enzyme encoded by the luciferase reporter gene catalyzes the oxidation of D-luciferin in the presence of ATP, oxygen, and Mg2+. The fluorescent product is quantified to measure its activity.

Pictogrammes

CorrosionEnvironment
GHS05,GHS09

Mention d'avertissement

Danger

Mentions de danger

H318,H410

Classification des risques

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Sigma-Aldrich

SCT149

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Articles

Bioluminescent Firefly Luciferase Assays

Firefly luciferase is a widely used bioluminescent reporter for studying gene regulation and function. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.

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