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L8795

Sigma-Aldrich

LB Agar Tetracycline-10, Plates

pre-poured agar plates with 10 μg/ml tetracycline

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About This Item

Code UNSPSC :
12352200

Qualité

for molecular biology

Stérilité

sterile; autoclaved

Forme

plates (10cm, 20-25ml each)

Disponibilité

available only in USA

Température de stockage

2-8°C

Adéquation

selective for Escherichia coli
selective for coliforms

Description générale

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for selective cultivation of E. coli strains that contain plasmids conferring tetracycline resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins.

Caractéristiques et avantages

Miller′s LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation plus antibiotic

Composants

15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
10μg/ml tetracycline

Autres remarques

The plates must be used within two months of the manufacture date to ensure the antibiotics are fully active. The product may be shipped up until its expiration date but is generally shipped 2-4 weeks before expiration.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

T7660

Tetracycline hydrochloride, powder, BioReagent, suitable for cell culture

L3147

LB Broth with agar (Miller), Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.

L5542

LB Agar Plates, pre-poured LB agar plates for E. coli

L5667

LB Agar Ampicillin-100, Plates, pre-poured LB agar plates with 100 μg/ml ampicillin

L0418

LB Agar Carbenicillin - 100, Plates, pre-poured agar plates with 100 μg/ml carbenicillin

L0543

LB Agar Kanamycin-50, Plates, pre-poured LB agar plates with 50 ug/ml kanamycin

L0168

LB Agar Ampicillin - 50, Plates, pre-poured LB agar plates with 50 μg/ml ampicillin

CMC0001

SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production

CMC0007

SIG10 5a Chemically Competent Cells, for DNA plasmid production

CMC0014

BL21(DE3) Chemically Competent Cells, for protein expression

CMC0017

OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Tetracycline hydrochloride ≥95% (European Pharmacopoeia HPLC assay)

Sigma-Aldrich

T3383

Tetracycline hydrochloride

Bouillon LB (Miller) Liquid microbial growth medium

Sigma-Aldrich

L2542

Bouillon LB (Miller)

LB Broth with agar (Miller) Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.

Sigma-Aldrich

L3147

LB Broth with agar (Miller)

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Why do the LB-agar plates with antibiotics have such a short expiration date?

    The antibiotics in the LB-agar plates are not stable.  The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate.  Because of this, we recommend the plates are used as soon as possible once they are received.  For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).

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    Ask a Scientist here.

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