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H8416

Sigma-Aldrich

Stem Cell Factor human

≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

Synonyme(s) :

SCF, c-Kit ligand

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About This Item

Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

product name

Stem Cell Factor human, SCF, recombinant, expressed in HEK 293 cells, suitable for cell culture

Source biologique

human

Niveau de qualité

Produit recombinant

expressed in HEK 293 cells

Pureté

≥95% (SDS-PAGE)

Forme

lyophilized powder

Puissance

≤200 ng/mL ED50

Qualité

endotoxin tested

Poids mol.

dimer 35-45 kDa (glycosylated)

Conditionnement

pkg of 5x10 μg
pkg of 10 μg
pkg of 100 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤1 EU/μg

Numéro d'accès UniProt

Température de stockage

−20°C

Informations sur le gène

human ... KITLG(4254)

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Description générale

The KITLG (KIT ligand) gene is mapped to human chromosome 12q21.32.

Actions biochimiques/physiologiques

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.
Stem cell factor (SCF) of granulosa cell origin, efficiently contributes to the follicular development and oocyte maturation. SCF controls tumor development and progression. Elevated SCF gene expression is observed in hepatocellular carcinoma.

Notes préparatoires

SCF is expressed in human HEK 293 cells as a glycosylated monomer with an apparent molecular mass of 35-45 kDa due to glycosylation. Production in human 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Remarque sur l'analyse

The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Informations légales

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Increased SCF in Follicular Fluid and Granulosa Cells Positively Correlates With Oocyte Maturation, Fertilization, and Embryo Quality in Humans.
Tan J, et al.
Reproductive Sciences, 24(11), 1544-1550 (2017)
Gene mapping study for constitutive skin color in an isolated Mongolian population
Paik SH, et al.
Experimental & Molecular Medicine, 44, 241-249 (2012)
Yoshimichi Okayama et al.
Immunologic research, 34(2), 97-115 (2006-06-09)
Mast cells play a pivotal role in immediate hypersensitivity and chronic allergic reactions that can contribute to asthma, atopic dermatitis, and other allergic diseases. Because mast cell numbers are increased at sites of inflammation in allergic diseases, pharmacologic intervention into
Prabir Ray et al.
Cell cycle (Georgetown, Tex.), 7(18), 2826-2832 (2008-09-13)
The receptor tyrosine kinase, c-kit, and its ligand, stem cell factor (SCF), function in a diverse range of biological functions. The role of c-kit in the maintenance and survival of hematopoietic stem cells and of mast cells is well recognized.
Sibylle A Brenner et al.
Immunology, 143(2), 174-183 (2014-04-05)
Mast cells are now considered sentinels in immunity. Given their location underneath the gastrointestinal barrier, mast cells are entrusted with the task of tolerating commensal microorganisms and eliminating potential pathogens in the gut microbiota. The aim of our study was

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