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G5035

Sigma-Aldrich

Granulocyte-Macrophage Colony-Stimulating Factor human

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonyme(s) :

GM-CSF human

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About This Item

Numéro MDL:
MFCD00166611
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

product name

Granulocyte-Macrophage Colony-Stimulating Factor human, GM-CSF, recombinant, expressed in E. coli, suitable for cell culture

Source biologique

human

Niveau de qualité

200

Produit recombinant

expressed in E. coli

Pureté

≥98% (SDS-PAGE)

Forme

lyophilized powder

Puissance

≤0.1 ng/mL EC50

Poids mol.

14.6 kDa

Conditionnement

pkg of 4X5 μg
pkg of 5 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤1.000 EU/μg

Couleur

white

Solubilité

water: soluble 0.010 mL, clear, colorless

Numéro d'accès UniProt

P04141

Température de stockage

−20°C

Informations sur le gène

human ... CSF2(1437) , CSF2(1437)

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Description générale

Package size based on protein content

Actions biochimiques/physiologiques

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

Forme physique

Lyophilized from a 0.2 μm filtered, 10 mM sodium citrate, pH 3.5 solution.

Remarque sur l'analyse

The proliferative activity of human GM-CSF is tested in culture using human TF-1 cells.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Benhur Lee et al.
Research square (2021-10-07)
Bats are significant reservoir hosts for many viruses with zoonotic potential1. SARS-CoV-2, Ebola virus, and Nipah virus are examples of such viruses that have caused deadly epidemics and pandemics when spilled over from bats into human and animal populations2,3. Careful
Kazuhiro Maki et al.
International journal of hematology, 100(4), 361-369 (2014-07-25)
Myelodysplastic syndrome (MDS) is a stem cell tumor characterized by dysplastic features and ineffective hematopoiesis in the early phase and leukemic progression in the late phase. Speculating that differences in the expression of genes and microRNA (miRNA) in control and
Antonella Mancusi et al.
Blood, 125(20), 3173-3182 (2015-03-15)
Because activating killer cell immunoglobulinlike receptors (KIRs) are heterogeneously expressed in the population, we investigated the role of donor activating KIRs in haploidentical hematopoietic transplants for acute leukemia. Transplants were grouped according to presence vs absence of KIR-ligand mismatches in
Fengjiao Yuan et al.
PloS one, 9(9), e107063-e107063 (2014-09-10)
Cigarette smoking is a major pathogenic factor in lung cancer. Macrophages play an important role in host defense and adaptive immunity. These cells display diverse phenotypes for performing different functions. M2 type macrophages usually exhibit immunosuppressive and tumor-promoting characteristics. Although
T Kitamura et al.
Journal of cellular physiology, 140(2), 323-334 (1989-08-01)
We have established a novel cell line, designated as TF-1, from a patient with erythroleukemia, which showed complete growth dependency on granulocyte-macrophage colony-stimulating factor (GM-CSF) or on interleukin-3 (IL-3) and carried a homogeneous chromosomal abnormality (54X). Erythropoietin (EPO) also sustained
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