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Key Documents

GE29-0513-25

HiTrap® Q HP

Cytiva 29-0513-25, pack of 1 mL

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About This Item

Code UNSPSC :
23151816
Nomenclature NACRES :
NA.56

ligand

quaternary amine

Description

Ion Exchanger Type (value)

Conditionnement

pack of 1 mL

Fabricant/nom de marque

Cytiva 29-0513-25

Disponibilité

not available in North America

Paramètres

42 psi

Taille du lit

7 mm × 25 mm

Volume du lit

1 mL

D.I. de la colonne

7 mm

Matrice

6% cross-linked agarose

Taille des particules

24-44 μm

Taille moy. des particules

34 μm

cleaning

1-14

working range

2-12

Adéquation

suitable for bioprocess medium

Catégories apparentées

Description générale

HiTrap Q HP are prepacked, ready-to-use Q Sepharose High Performance strong anion exchange columns for high-resolution, small-scale protein purification.

Application

Separation of protein using a strong anion exchange medium prepacked in the HiTrap column format.

Caractéristiques et avantages

  • 34 μm bead size for high-performance, high-resolution purifications.
  • Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
  • Designed for use with a syringe, peristaltic pump, and chromatography systems such as AKTA design.
  • Strong quaternary ammonium (Q) anion exchanger.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

HiTrap is a registered trademark of Cytiva

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

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Articles

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocoles

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

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