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GE28-9440-06

Protein A Mag Sepharose™

Cytiva 28-9440-06, pack of 1 × 500 μL

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

native protein A

Conditionnement

pack of 1 × 500 μL

Fabricant/nom de marque

Cytiva 28-9440-06

Conditions de stockage

(20% Ethanol, 20% medium slurry)

Paramètres

Room temperature temp. range

Matrice

paramagnetic, spherical, highly cross-linked agarose particles

Capacité

8- 17 mg binding capacity(human IgG/ml gel)

Température de stockage

2-8°C

Catégories apparentées

Description générale

Protein A Mag Sepharose™ are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. Based on Sepharose™ with native Protein A as ligand.

Application

Protein A Mag Sepharose™ are magnetic beads designed to simplify enrichment of target Proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose™ with native Protein A as ligand. Protein A has high affinity for the Fc region of IgG antibodies from a broad variety of species.

There are two protocols available for Protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for both electrophoresis and MS analysis.

Protein A Mag Sepharose™ combines well-proven enrichment methods with the magnetic beads platform, which is highly suitable for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.

Caractéristiques et avantages

  • Visible and dense Sepharose™ based magnetic beads with Protein A ligand, easy to spot and collect bound target Protein
  • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
  • Simple capture of target Protein in small or large sample volumes (Low microliter to high milliliter scale)
  • Optimized capacity for enrichment/immunoprecipitation of target Proteins - only Low amounts of antibody needed
  • Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Protocoles

Performing a Purification of IgG Antibodies with Protein A Mag Sepharose® Xtra or Protein G Mag Sepharose® Xtra

This page shows how to separate IgG antibodies by affinity chromatography using Protein A Mag Sepharose Xtra or Protein G Mag Sepharose Xtra from GE Healthcare.

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