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Key Documents

GE17-1279-01

rProtein A Sepharose™ Fast Flow

Cytiva 17-1279-01, pack of 5 mL

Synonyme(s) :

Fast Flow Sepharose, Protein A Sepharose

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

recombinant protein A (E. coli)

Conditionnement

pack of 5 mL

Fabricant/nom de marque

Cytiva 17-1279-01

Conditions de stockage

(20% Ehtanol)

Matrice

4% cross-linked agarose

Taille des particules

60-165 μm

Diamètre moyen

90 μm (d50v)

cleaning in place

2-11

working range

3-10

Capacité

~50 mg binding capacity (human lgG/ml medium)

Adéquation

suitable for bioprocess medium

Température de stockage

2-8°C

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Shuai Liu et al.
Rice (New York, N.Y.), 12(1), 86-86 (2019-11-30)
Histone modifications play important roles in growth and development of rice (Oryza sativa L.). Lysine butyrylation (Kbu) with a four-carbon chain is a newly-discovered histone acylation modification in rice. In this study, we performed chromatin immunoprecipitation sequencing (ChIP-seq) analyses, the

Articles

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Protocoles

Performing a Separation with GE Healthcare Products Based on Protein A

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from GE Healthcare.

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from GE Healthcare.

Converting From Linear Fow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

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