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Key Documents

GE28-9365-49

HiPrep™ Heparin Fast Flow 16/10

Cytiva 28-9365-49

Synonyme(s) :

Heparin affinity chromatography

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About This Item

Code UNSPSC :
13111000
Nomenclature NACRES :
NA.56

ligand

porcine heparin

Forme

prepacked column

Réticulation

cross-linked

Conditionnement

pkg of 1 ea

Fabricant/nom de marque

Cytiva 28-9365-49

Conditions de stockage

(20% ethanol containing 0.05 M sodium acetate)

Paramètres

1.5 bar (22 psi) (Over the Packed Bed During Operation)
2 ml/min - 10 ml/min flow rate
22 psi

Technique(s)

liquid chromatography (LC): suitable

Taille du lit

16 mm × 100 mm

Volume du lit

20 mL

D.I. de la colonne

16 mm

Matrice

6% cross-linked agarose

Groupe de la matrice active

heparin

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning

4-13

working range

4-12

Adéquation

suitable for bioprocess medium

Technique de séparation

affinity

Description générale

HiPrep™ Heparin FF 16/10 is prepacked with Heparin Sepharose™ 6 Fast FLow for preparative purification of proteins with affinity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.

Application

The column provides fast, preparative separations of Proteins and other biomolecules. Immobilized heparin has two main modes of interaction with Proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma Proteins, lipoProteins, Protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high-capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases. Heparin is coupled to Sepharose™ 6 Fast FLow with a chemically optimized linkage.

Heparin is a mucopolysaccharide consisting of a repeating dimer of α-L-dipyranuronic acid 2-sulfate, and 2-deoxy-2-sulfamino-α-D-glucopyranose 6-sulfate, with a molecular weight range of 5 000 to 30 000.

Caractéristiques et avantages

  • 20 mL column optimized for convenient scale-up purification .
  • Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma Proteins.
  • Simple operation with single pump configurations, as well as ÄKTA® design systems.
  • Excellent flow characteristics.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.05 M Sodium Acetate containing 20% Ethanol)
Store at 4 to 30 °C (20% Ethanol, 0.05 M Sodium Acetate)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

HiPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocoles

Purification or Removal of DNA-Binding Proteins

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Performing a Separation of DNA binding proteins with GE Healthcare Products Based on Heparin

DNA binding proteins form an extremely diverse class of proteins sharing a single characteristic, their ability to bind to DNA. Functionally the group can be divided into those responsible for the replication and orientation of the DNA such as histones, nucleosomes and replicases and those involved in transcription such as RNA/DNA polymerases, transcriptional activators and repressors and restriction enzymes.

Performing a Separation with GE Healthcare Products Based on GST Fusion Proteins

How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

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