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GE17-5318-06

Ni Sepharose™ 6 Fast Flow

Cytiva 17-5318-06, pack of 5 mL

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

Conditionnement

pack of 5 mL

Fabricant/nom de marque

Cytiva 17-5318-06

Matrice

6% cross-linked agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning in place

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

Capacité

~40 mg binding capacity(histidine-tagged protein)

Adéquation

suitable for bioprocess medium

Description générale

Ni Sepharose™ 6 Fast FLow is a BioProcess medium that combines the advantages of Ni?? for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose™ Fast FLow platform.

Application

Ni Sepharose™ 6 Fast FLow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.

Ni Sepharose™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2? ions for purification of histidine tagged Proteins.

The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2? leakage while providing high capacity.

Ni Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Ni Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Caractéristiques et avantages

  • Designed for scaling up purification of histidine-tagged Proteins
  • High Protein binding capacity and minimal leakage of Ni2? ions
  • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in convenient prepacked HisPrep™ and HisTrap™ column formats.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
HisPrep is a trademark of Cytiva
HisTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogrammes

Environment
GHS09

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Pär Comstedt et al.
PloS one, 9(11), e113294-e113294 (2014-11-20)
There is currently no Lyme borreliosis vaccine available for humans, although it has been shown that the disease can be prevented by immunization with an OspA-based vaccine (LYMErix). Outer surface protein A (OspA) is one of the dominant antigens expressed

Articles

Performing High-Throughput Screening Using His MultiTrap™ HP and His MultiTrap™ FF 96-Well filter Plates

This page shows high-throughput screening using His MultiTrap HP and His MultiTrap FF 96-well filter plates from GE Healthcare.

Performing a Cell Lysis of Recombinant Histidine-Tagged Proteins

This page shows a general procedure for cell lysis of recombinant histidine-tagged proteins using GE Healthcare products.

Protocoles

Manual Purification Using HisTrap FF Crude Kit with a Syringe

This page shows how to purify histidine-tagged recombinant proteins manually with a syringe using HisTrap FF crude Kit from GE Healthcare.

Gravity-flow Purification using His GraviTrap™ and His GraviTrap™ Kit

This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from GE Healthcare.

Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using HisTrap™ Excel

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from GE Healthcare.

Purification from Unclarified Cell Lysate using HisTrap™ FF Crude

This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from GE Healthcare.

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