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GE17-0980-01

Butyl Sepharose™ 4 Fast Flow

Cytiva 17-0980-01, pack of 200 mL

Synonyme(s) :

Butyl Sepharose, Fast Flow Sepharose

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

butyl

Conditionnement

pack of 200 mL

Fabricant/nom de marque

Cytiva 17-0980-01

Matrice

4% cross-linked agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning in place

2-14

working range

3-13

Adéquation

suitable for bioprocess medium

Catégories apparentées

Description générale

Butyl Sepharose™ 4 Fast FLow is a well established, standard aliphatic hydrophobic interaction chromatography (HIC) medium for capture and intermediate purification of larger proteins.

Application

Butyl Sepharose™ 4 Fast FLow is part of the Sepharose™ Fast FLow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.
Butyl Sepharose™ 4 Fast FLow is based on cross-linked 4% Agarose. The aliphatic ligand is immobilized to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.

Butyl Sepharose™ 4 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Butyl Sepharose™ 4 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Caractéristiques et avantages

  • Standard aliphatic HIC media based on Sepharose™ Fast FLow base matrix derivatized via uncharged, chemically-stable ether linkages.
  • Optimized for the separation of larger Proteins in capture and intermediate purification steps
  • BioProcess medium supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

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Articles

Salt Selection and Buffer Preparation

This section is about salt selection and buffer preparation in HIC medium from GE Healthcare, parameters such as buffer ions and pH are considered

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography

Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with GE Healthcare media are detailed, including sample application, load volume and temperature

Protocoles

Selectivity and the Properties of a HIC Medium

While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent cleaning conditions and their low level of non-specific interaction.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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