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GE17-0978-02

Butyl-s Sepharose™ 6 Fast Flow

Cytiva 17-0978-02, pack of 200 mL

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

butyl-S

Conditionnement

pack of 200 mL

Fabricant/nom de marque

Cytiva 17-0978-02

Matrice

6% cross-linked agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning in place

2-14

Plage de travail

3-13

Adéquation

suitable for bioprocess medium

Description générale

Butyl-S Sepharose™ 6 Fast FLow is the least hydrophobic medium in the Sepharose™ Fast FLow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of a purification process to remove the bulk of impurities.

Application

Butyl-S Sepharose™ 6 Fast FLow is part of the Sepharose™ Fast FLow HIC platform, which has been an industrial standard for HIC chromatography during recent decades. Butyl-S Sepharose™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with butyl groups via a thio-ether linkage. It will exhibit a different selectivity compared to Butyl Sepharose™ 4 Fast FLow and other HIC Sepharose™ Fast FLow products.

This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively Low salt concentrations. Butyl?S Sepharose™ 6 Fast FLow is particularly useful during the initial stages of a separation process to remove the bulk of impurities without stringent requirements for conditioning of the sample (e.g. adjustment of pH, salt concentration etc.)

Butyl-S Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Butyl-S Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Caractéristiques et avantages

  • Binds and elutes relatively strong hydrophobic molecules at comparatively Low salt concentrations
  • The least hydrophobic medium in the well proven Sepharose™ Fast FLow HIC range
  • Purifies recombinant Hepatitis B virus surface antigen from CHO cells
  • Developed and optimized in cooperation with a process-scale manufacturer of biopharmaceuticals
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

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Certificats d'analyse (COA)

Lot/Batch Number

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Articles

Salt Selection and Buffer Preparation

This section is about salt selection and buffer preparation in HIC medium from GE Healthcare, parameters such as buffer ions and pH are considered

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography

Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with GE Healthcare media are detailed, including sample application, load volume and temperature

Protocoles

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

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