F9554

Anti-Fatty Acid Synthase antibody produced in rabbit

1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-FAS, Anti-FASN
• Numéro MDL: MFCD02262888
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 270 kDa
• Espèces réactives: mouse, rat, human (predicted)
• Concentration: 1 mg/mL
• Technique(s): western blot: 0.5-1 μg/mL using lysates of mouse myoblast C2 cells; western blot: 0.5-1 μg/mL using lysates of rat hepatocytoma FAO cells
• Numéro d'accès UniProt: P49327
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... FASN(2194) ; mouse ... Fasn(14104) ; rat ... Fasn(50671)

Description

Description générale

Fatty acid synthase (FAS, FASN) is encoded by the gene mapped to human chromosome 17q25.3.

Fatty acid synthase (FAS, FASN) is encoded by the gene mapped to human chromosome 17q25.3. In mammals, it is composed of two identical multifunctional polypeptides, in which three catalytic domains in the N-terminal section (β-ketoacyl synthase, malonyl/acetyl tansferase, and dehydrase) are separated by a core region of about 600 residues from four C-terminal domains (enoyl reductase, β-ketoacyl reductase, acyl carrier protein, and thioesterase).

Immunogène

synthetic peptide corresponding to amino acids 1982-1999 of mouse fatty acid synthase, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is conserved in rat and differs from the human sequence by three amino acids.

Application

Anti-Fatty Acid Synthase antibody is suitable for western blot analysis at a concentration of 0.5-1μg/mL using lysates of mouse myoblast C2 cells and rat hepatocytoma FAO cells.

Fatty acid synthase antibody produced in rabbit has been used in immunohistochemistry and western blot analysis.

Actions biochimiques/physiologiques

FAS plays an essential role during embryogenesis and energy homeostasis in adult animals. It also acts as the carbon source for fatty acid synthesis. It has been shown to be overexpressed in various types of human cancer, including prostate, breast, ovary and others. FASN is a multifunctional protein, mainly involved in catalyzing the synthesis of palmitate from acetyl-CoA and malonyl CoA, in the presence of NADPH.

Description de la cible

Fatty Acid Synthase encodes an enzyme that is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)