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Key Documents

P0290

Sigma-Aldrich

Phenol red solution

0.5%, liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonyme(s) :

Phenolsulfonphthalein solution

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About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352207
ID de substance PubChem :
Nomenclature NACRES :
NA.75

Stérilité

sterile-filtered

Niveau de qualité

Gamme de produits

BioReagent

Forme

liquid

Concentration

0.5%

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Chaîne SMILES 

O=S1(C2=CC=CC=C2C(C3=CC=C(O)C=C3)(C4=CC=C(O)C=C4)O1)=O

InChI

1S/C19H14O5S/c20-15-9-5-13(6-10-15)19(14-7-11-16(21)12-8-14)17-3-1-2-4-18(17)25(22,23)24-19/h1-12,20-21H

Clé InChI

BELBBZDIHDAJOR-UHFFFAOYSA-N

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Description générale

Phenol red belongs to the class of triphenylmethane dyes and is widely used in the textile industry.

Application

Phenol red solution has been used:
  • as a tracer dye for microinjection of zebrafish eggs
  • as a component of the mMW medium for the preparation of mouse embryos
  • in the preparation of versene-ethylenediamine tetraacetic acid (EDTA) solution
  • as a pH indicator in Dulbecco′s modified Eagle medium (DMEM) during glycolytic stress (GST) tests and mitochondrial stress tests (MST) to evaluate metabolic function in cells.

Actions biochimiques/physiologiques

Phenol red solution acts as a pH indicator for the culture medium.Phenol red solution acts as a pH indicator for the culture medium that turns yellow at a pH of 6.4 or lower and changes into red at a pH of 8.2 or higher. It enables a fast and simple assessment of the culture′s health. In addition to its application as a pH indicator in culture mediums, Phenol red is used to detect human peroxidase enzyme activity, as well as cellular hydrogen peroxide levels, in various experiments.

Caractéristiques et avantages

High-quality, endotoxin-tested solution, suitable for mammalian cell culture.

Notes préparatoires

Prepared in Dulbecco′s Phosphate Buffered Saline.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Shivshankar R Mane et al.
Small (Weinheim an der Bergstrasse, Germany), 14(36), e1801571-e1801571 (2018-08-07)
Recent advances in super-resolution microscopy and fluorescence bioimaging allow exploring previously inaccessible biological processes. To this end, there is a need for novel fluorescent probes with specific features in size, photophysical properties, colloidal and optical stabilities, as well as biocompatibility
Natacha Merindol et al.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 128, 104423-104423 (2020-05-18)
Rapid and reliable screening of SARS-CoV-2 is fundamental to assess viral spread and limit the pandemic we are facing. In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar® SARS-CoV-2 rRT-PCR
S F Gabby Krens et al.
BMC genomics, 9, 196-196 (2008-04-30)
The MAPK signaling proteins are involved in many eukaryotic cellular processes and signaling networks. However, specific functions of most of these proteins in vertebrate development remain elusive because of potential redundancies. For instance, the upstream activation pathways for ERK1 and
Ngoc Tung Tran et al.
STAR protocols, 1(1), 100028-100028 (2020-07-21)
Mutations that accumulate in self-renewing hematopoietic stem and progenitor cells (HSPCs) can cause severe blood disorders. To model such disorders in mice, we developed a CRISPR/Cas9/adeno-associated virus (AAV)-based system to knock in and repair genes by homologous recombination in mouse
Maximiliano Tourmente et al.
Reproduction (Cambridge, England), 154(4), 341-354 (2017-07-06)
Whereas a broad link exists between nucleotide substitutions in the mitochondrial genome (mtDNA) and a range of metabolic pathologies, exploration of the effect of specific mtDNA genotypes is on-going. Mitochondrial DNA mutations are of particular relevance for reproductive traits, since

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