ED4SS

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate

99.0-102.0% (titration)

• Synonyme(s) : EDTA tetrasodium salt, Edathamil, Tetrasodium ethylenediaminetetraacetate dihydrate
• Formule linéaire : (NaOOCCH₂)₂NCH₂CH₂N(CH₂COONa)₂ · 2H₂O
• Numéro CAS: 10378-23-1
• Poids moléculaire : 416.20
• Numéro Beilstein : 3861753
• Numéro CE : 200-573-9
• Numéro MDL: MFCD00150026
• Code UNSPSC : 12352202
• ID de substance PubChem : 24894711
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 300
• Pureté: 99.0-102.0% (titration)
• Forme: crystalline
• Pertinence de la réaction: reagent type: chelator
• Solubilité: H₂O: soluble, clear, colorless
• Chaîne SMILES: O.O.[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C10H16N2O8.4Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;;2*1H2/q;4*+1;;/p-4
• Clé InChI: KSYNLCYTMRMCGG-UHFFFAOYSA-J

Description

Description générale

Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid and a metal chelating agent. It comprises four carboxylic acid and two amine groups with lone-pair electrons that can chelate calcium and several other metal ions. 

Application

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate has been used:

• in the decalcification of femurs for the histoprocessing and staining of osteochondral tissue
• as a component of NH₄Cl lysis buffer for erythrocyte lysis and bulk characterization
• for the isolation of crypts from the connective tissues of mice

Actions biochimiques/physiologiques

Ethylenediaminetetraacetic acid (EDTA) is used to eliminate enzyme inhibition by traces of heavy metals, and to inhibit enzymes that require divalent cations as cofactors.

Ethylenediaminetetraacetic acid (EDTA) is used to treat patients poisoned with heavy metal ions. It functions as a chelator of the zinc ion in the active site of metalloproteases. EDTA inhibits other metal ion-dependent proteases such as calcium-dependent cysteine proteases. It might interfere with biological events which are metal ion-dependent. EDTA inhibits platelet aggregation and is the most preferred anticoagulant for platelet counts. It associates with other active agents to modulate microorganisms and biofilms, like citric acid, alcohol, antibiotics, polyhexamethylene biguanide (PHMB) quaternary ammonium compounds, and other antiseptics. EDTA is used as an anticoagulant for hematological testing, as it preserves the cellular components and morphology of blood cells. 

Informations sur la sécurité

• Pictogrammes: GHS08,GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H302 + H332,H318,H373
• Conseils de prudence: P260 - P280 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Classification des risques: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - STOT RE 2
• Organes cibles: Respiratory Tract
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves