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E8656

Sigma-Aldrich

Heat-Labile Enterotoxin, B subunit from E. coli

recombinant, expressed in Pichia pastoris, >90% (SDS-PAGE), lyophilized powder

Synonyme(s) :

LTB

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77

Produit recombinant

expressed in Pichia pastoris

Niveau de qualité

200

Pureté

>90% (SDS-PAGE)

Forme

lyophilized powder

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Application

Heat-Labile Enterotoxin, B subunit (LTB) from E. coli has been used in enzyme-linked immunosorbent assay (ELISA).

Actions biochimiques/physiologiques

Enterotoxigenic Escherichia coli causes diarrhea through its heat-labile enterotoxin (LT). The LT is a periplasmic protein composed of one A subunit (LTA, 27 kDa) and five non-covalently associated B subunits (LTB, 11.6 kDa each) forming a ring-like pentamer. LTB has high affinity towards the toxin receptor ganglioside GM1, a glycosphingolipid found ubiquitously on the surface of mammalian cells. Ganglioside GM1 facilitates the delivery of the A subunit to the cytosol of the target cell resulting in persistent synthesis of cAMP and subsequently diarrhea. This characteristic makes LTB a good label for microglial cells (due to the enrichment of ganglioside GM1 on their cell surface). In addition, studies made mostly with animal models demonstrated that recombinant LTB could stimulate strong serum and mucosal immune responses against LT. Other studies have indicated that LTB could be used as a potent mucosal adjuvant.

Caractéristiques et avantages

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Forme physique

Lyophilized from a solution containing Heat-Labile Enterotoxin, B subunit in 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3, and 1 mM sodium EDTA.

Remarque sur l'analyse

The activity is measured by ELISA using ganglioside GM1-coated plates (Cat. No. G7641). LTB at various concentrations is incubated on the ganglioside GM1 coated wells, followed by anti-cholera toxin antibody (Cat. No. C3062), and peroxidase-labeled goat anti-rabbit IgG (Cat.No. A0545) as the secondary antibody. Binding saturation of 50% may be achieved with = 0.12 μg/mL of LTB.

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H312 - H412

Classification des risques

Acute Tox. 4 Dermal - Aquatic Chronic 3

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Safety and immunogenicity of escalating dosages of a single oral administration of peru-15 pCTB, a candidate live, attenuated vaccine against enterotoxigenic Escherichia coli and Vibrio cholerae
Chen WH, et al.
Clinical and Vaccine Immunology : CVI, 22(1) (2015)
Preformulation Characterization and Stability Assessments of Secretory IgA Monoclonal Antibodies as Potential Candidates for Passive Immunization by Oral Administration
Hu Y, et al.
Journal of Pharmaceutical Sciences, 109(1) (2020)
Sandra Scheiblhofer et al.
Vaccine, 39(32), 4399-4403 (2021-07-07)
The skin represents an attractive target tissue for vaccination against respiratory viruses such as SARS-CoV-2. Laser-facilitated epicutaneous immunization (EPI) has been established as a novel technology to overcome the skin barrier, which combines efficient delivery via micropores with an inherent
Plastid expression of a double-pentameric vaccine candidate containing human papillomavirus-16 L1 antigen fused with LTB as adjuvant: transplastomic plants show pleiotropic phenotypes
Waheed MT, et al.
Plant Biotechnology Journal, 9(6) (2011)

Contenu apparenté

Discover Bioactive Small Molecules for Cyclic Nucleotides

Cyclic nucleotides, including cyclic AMP (cAMP), cyclic GMP (cGMP) and cyclic ADP-ribose, have been extensively studied as second messengers of intracellular events initiated by activation of GPCRs. cAMP modifies cell function in all eukaryotic cells, principally through the activation of cAMP-dependent protein kinase (PKA), but also through cAMP-gated ion channels and guanine nucleotide exchange factors directly activated by cAMP.

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