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Key Documents

E8406

Sigma-Aldrich

Anti-< EDEM1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-EDEM, Anti-ER degradation enhancer

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen 65 kDa (nonglycosylated human and mouse EDEM1)
antigen 75 kDa

Espèces réactives

human, mouse

Technique(s)

immunoprecipitation (IP): 0.5-1.0 μg using extract HEK-293T cells expressing recombinant mouse EDEM1 protein.
western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM1.

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... EDEM1(9695)
mouse ... Pvalb(19293)

Description générale

Anti-EDEM1 recognizes glycoslylated human and mouse EDEM1 with molecular weight 75 kDa and nonglycosylated human and mouse EDEM1 with a molecular
weight 65 kDa.
ER degradation-enhancing alpha-mannosidase-like 1 (EDEM1) is an enzyme encoded by the EDEM1 gene in humans. In mammalian cells, EDEM1 is localized to the endoplasmic reticulum (ER), mainly as a soluble glycoprotein.

Immunogène

synthetic peptide corresponding to amino acids 629-646 of human EDEM1, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence is identical in mouse EDEM1.

Application

Anti-EDEM1 antibody has been used in immunoblotting.
Anti-EDEM1 antibody produced in rabbit is suitable for the following applications:
  • Immunoprecipitation at a concentration of 0.5-1.0μg using extract HEK-293T cells expressing recombinant mouse EDEM1 protein.
  • Western blotting at a concentration of 0.5-1.0μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM1.
  • Immunofluorescence Microscopy.
  • Immunoblotting
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Actions biochimiques/physiologiques

ER degradation-enhancing alpha-mannosidase-like 1 (EDEM1), a putative mannose binding lectin, targets misfolded glycoproteins for degradation in an N-glycan dependent manner. It interacts with the C-terminus of calnexin and lacks mannosidase activity.
ER degradation-enhancing alpha-mannosidase-like 1 is an enzyme encoded by the EDEM1 gene in humans. It is a chaperone for rod opsin and its expression can be used to promote correct folding enhancing degradation of mutant proteins in the ER to combat protein-misfolding disease. The endogenous EDEM1 in cells that are not stressed by the expression of a transgenic misfolded protein reaches the cytosol and is degraded by basal autophagy. EDEM may maintain the retrotranslocation competence of NHK by inhibiting aggregation. It is also considered responsible for directing aberrant proteins for ERAD (ER-associated protein degradation).

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

EDEM1 recognition and delivery of misfolded proteins to the SEL1L-containing ERAD complex
Cormier J H, et al.
Molecular Cell, 34(5), 627-633 (2009)
Sujin Park et al.
Histochemistry and cell biology, 142(2), 153-169 (2014-03-26)
ER degradation-enhancing α-mannosidase-like 1 protein (EDEM1) is involved in the routing of misfolded glycoproteins for degradation in the cytoplasm. Previously, we reported that EDEM1 leaves the endoplasmic reticulum via non-COPII vesicles (Zuber et al. in Proc Natl Acad Sci USA
Redundant and antagonistic roles of XTP3B and OS9 in decoding glycan and non-glycan degrons in ER-associated degradation
van der Goot A T, et al.
Molecular Cell, 70, 516-530 (2018)
Iryna Monastyrska et al.
Autophagy, 9(2), 164-174 (2012-11-28)
Equine arteritis virus (EAV) is an enveloped, positive-strand RNA virus. Genome replication of EAV has been associated with modified intracellular membranes that are shaped into double-membrane vesicles (DMVs). We showed by immuno-electron microscopy that the DMVs induced in EAV-infected cells
Fulvio Reggiori et al.
Cell host & microbe, 7(6), 500-508 (2010-06-15)
Coronaviruses (CoV), including SARS and mouse hepatitis virus (MHV), are enveloped RNA viruses that induce formation of double-membrane vesicles (DMVs) and target their replication and transcription complexes (RTCs) on the DMV-limiting membranes. The DMV biogenesis has been connected with the

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