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11718096001

Roche

DAB Substrate

dark brown, /black precipitate; Visually evaluated

Synonyme(s) :

3,3′-diaminobenzidine tetrahydrochloride, DAB

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About This Item

Code UNSPSC :
12352200

Fabricant/nom de marque

Roche

Niveau de qualité

100

Couleur

dark brown, /black precipitate; Visually evaluated

Température de stockage

−20°C

Catégories apparentées

Description générale

The metal enhanced DAB Substrate utilizes cobalt chloride and nickel chloride in a special formulation to produce a dark brown/black precipitate in the presence of horseradish peroxidase (HRP).
3, 3′ diaminobenzidine tetrahydrochloride (DAB) is a carcinogen that should be used safely. It is one of the major sensitive substrates for HRP (horseradish peroxidase). DAB is brown or amber in color and becomes insoluble and precipitates over the antigen-antibody site due to the presence of hydrogen peroxide and peroxidase.

Application

  • DAB Substrate is a color sustrate for peroxidases and is used in a variety of applications:Immunohistocytochemistry
  • In situ hybridization
  • Western blot
  • Dot blot
DAB substrate has been used in immunofluorescence and western blot.

Conditionnement

1 set containing 2 components.

Notes préparatoires

Working solution:
  1. Determine the volume of substrate required for the development of peroxidase.
  2. Remove the DAB/metal concentrate (10x) from -15 to -25 °C storage.
    Note: Do not bring to 15 to 25 °C.
  3. Dilute the DAB/metal concentrate (10x) with the peroxide buffer to a 1x working solution. For example, if 5 ml of substrate are required, dilute 500 μl of the DAB/metal concentrate (10x) with 4.5 ml of the Peroxide buffer.
  4. Mix well.
  5. Add the 1x working solution to the tissue or blot until desired substrate development.
    Note: The 1x solution is stable over many hours.
    For best results, store the working solution at 2 to 8 °C when not using.

Storage conditions (working solution): 2 to 8 °C
For best results, store the working solution at 2 to 8 °C when not using.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Composants de kit seuls

Réf. du produit
Description
  • DAB/metal concentrate, 25 ml 10x concentrated
  • Peroxide Buffer, 250 ml

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 4 - Aquatic Chronic 2 - Carc. 1A - Carc. 1B - Flam. Liq. 2 Oral - Repr. 1B - Skin Sens. 1 Inhalation - STOT RE 2 - STOT SE 1

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 3

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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João Francisco Botelho et al.
Proceedings. Biological sciences, 281(1788), 20140765-20140765 (2014-06-27)
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Pathobiology of Human Disease: A Dynamic Encyclopedia of Disease Mechanisms (2014)
Adrenergic Receptor Protocols (2000)
The developmental origin of zygodactyl feet and its possible loss in the evolution of Passeriformes.
Botelho J F, et al.
Proc. Royal Soc. Lond. B., 281(1788), 20140765-20140765 (2014)
Principles and Reactions of Protein Extraction, Purification, and Characterization (2005)
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