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D8187

Sigma-Aldrich

JumpStart™ REDTaq® DNA Polymerase

Hot-start Taq enzyme with inert dye, 10X buffer included

Synonyme(s) :

Hot start DNA polymerase, Hot start Taq

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About This Item

Numéro MDL:
MFCD00166026
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.55

Niveau de qualité

200

Forme

liquid

Utilisation

sufficient for 250 reactions
 sufficient for 2500 reactions
 sufficient for 50 reactions

Caractéristiques

dNTPs included: no
hotstart

Concentration

1 unit/μL

Technique(s)

PCR: suitable

Couleur

red

Entrée

purified DNA

Adéquation

suitable for PCR

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

JumpStart™ REDTaq® DNA Polymerase is Sigma′s high performance Taq DNA Polymerase blended with JumpStart Taq antibody and an inert red dye tracer. Extensive testing with a variety of primers and templates indicates that the performance of JumpStart REDTaq DNA Polymerase is equivalent to, or better than, that of standard Taq polymerase.
Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.

Application

JumpStart™ REDTaq® DNA Polymerase has been used in polymerase chain reaction (PCR) for the amplification of:
  • insulin-enterotoxin ricin fusion gene (INS-RTB)
  • endothelial cells DNA derived from reverse transcribed RNA
  • leg genomic DNA from cricket flies
  • mitochondrial gene by conventional PCR
JumpStart™ REDTaq® DNA Polymerase is also suitable for DNA methylation analysis.

Caractéristiques et avantages

  • Reduces non-specific amplification
  • Increased target yield and specificity
  • Higher the amplification irrespective of the target concentration
  • Reduce set-up time and eliminate concerns associated with manual or wax hot start methods
  • Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred
  • Samples can be loaded directly onto an agarose gel for electrophoresis without loading buffers or tracking dyes
  • Assembled PCR reactions can be placed at room temperature for up to 2 hours

Conditionnement

The enzyme is provided with an optimized 10× reaction buffer.

Définition de l'unité

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Autres remarques

View more detailed information on JumpStart REDTaq enzymes at www.sigma-aldrich.com/hotstart.

Informations légales

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

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2′-Deoxyguanosine 5′-triphosphate trisodium salt solution, 10 mM in H2O

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2′-Deoxycytidine 5′-triphosphate disodium salt, 10 mM, aqueous solution

D8418

Diméthylsulfoxyde, for molecular biology

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Huile minérale, PCR Reagent

D6920

2′-Deoxyadenosine 5′-triphosphate sodium salt solution, 10 mM

B0300

Bétaïne solution, 5 M, PCR Reagent

W1754

Eau, PCR Reagent

D4184

Taq ADN polymérase JumpStart™, without MgCl2

P2893

JumpStart™ Taq ReadyMix™, Complete optimized reagent for hot-start PCR at 2X concentration

D7295

Mélange de désoxynucléotides, 10 mM, Molecular Biology Reagent

D9307

Taq ADN polymérase JumpStart™, with MgCl2

P1107

Mélange réactionnel JumpStart™ REDTaq® ReadyMix™, for High-throughput PCR of complex templates

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H315,H319,H335

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

The field cricket Gryllus assimilis and two new sister species (Orthoptera: Gryllidae).
Weissman, D.B., et al.
Annals of the Entomological Society of America, 102, 367-367 (2009)
Billions and billions sold: pet-feeder crickets (Orthoptera: Gryllidae), commercial cricket farms, an epizootic densovirus, and government regulations make for a potential disaster
Weissman DB, et al.
Zootaxa, 3504(1), 67-88 (2012)
James E Carter et al.
Molecular biotechnology, 44(2), 90-100 (2009-11-10)
Onset of juvenile Type 1 diabetes (T1D) occurs when autoreactive lymphocytes progressively destroy the insulin-producing beta-cells in the pancreatic Islets of Langerhans. The increasing lack of insulin and subsequent onset of hyperglycemia results in increased damage to nerves, blood vessels
Expression of a ricin toxin B subunit: insulin fusion protein in edible plant tissues
Carter JE, et al.
Molecular Biotechnology, 44(2), 90-100 (2010)
Contributions of VEGF to age-dependent transmural gradients in contractile protein expression in ovine carotid arteries
Butler SM, et al.
American Journal of Physiology. Cell Physiology, 301(3), C653-C666 (2011)
Afficher tous les articles scientifiques apparentés

Articles

Introduction and Historical Timelines

Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

Protocoles

Applications with REDTaq™

Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Antibody-Enzyme Mediated Hot Start PCR Protocol

When using hot start Taq DNA polymerase, the enzyme remains inactive until heated. Hot Start DNA polymerase control is achieved by chemical or antibody modification of the enzyme.

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