A7721
JumpStart™ Taq Antibody
Adds hot-start capabilities to any Taq DNA Polymerase
Synonyme(s) :
Hot start Taq antibody
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About This Item
Produits recommandés
Source biologique
mouse
Conjugué
unconjugated
Clone
monoclonal
Forme
liquid
Caractéristiques
hotstart
Concentration
1.1 mg/mL
Technique(s)
PCR: suitable
Couleur
colorless
Conditions d'expédition
wet ice
Température de stockage
−20°C
Catégories apparentées
Description générale
JumpStart™ Taq Antibody provides an efficient method for hot-start PCR. This antibody is a neutralizing monoclonal antibody specific to Taq DNA polymerase. When the antibody is bound to Taq, the enzyme is rendered inactive. At the first denaturation step in thermal cycling, the enzyme-antibody complex dissociates, and the JumpStart Taq Antibody becomes nonfunctional. Once the antibody is denatured, the activity of the Taq DNA polymerase is restored and the enzyme functions normally during the PCR reaction. JumpStart Taq Antibody is commonly used to prevent undesirable amplification products.
Application
JumpStart™Taq Antibody is suitable for use in:
- cytochrome blocked nucleic acid (LNA) probe assay
- ligation-mediated polymerase chain reaction (LM-PCR)
- in Real-Time quantitative PCR Analysis of viral titer
- PCR amplification of complex genomic or cDNA templates
- to amplify very low-copy-number targets
- for the PCR amplification of many thermal cycles (>35) and multiple primer pairs in the same reaction tube
Conditionnement
Supplied with dilution buffer.
Composants
JumpStart Taq Antibody, 1.1μg/1μL
Dilution Buffer provided in 1 mL vials
Dilution Buffer provided in 1 mL vials
Quantité
Two units of Taq DNA polymerase are inactivated by 1 test of JumpStart Taq Antibody.
Autres remarques
JumpStart™ Taq Antibody is for R&D use only.Not for drug, household, or other uses.
View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.
View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.
Informations légales
JumpStart is a trademark of Sigma-Aldrich Co. LLC
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves
Certificats d'analyse (COA)
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D J Sharkey et al.
Bio/technology (Nature Publishing Company), 12(5), 506-509 (1994-05-01)
We demonstrate the utility of antibodies to the DNA polymerase from Thermus aquaticus (TaqPol) as thermolabile inhibitors of TaqPol activity. One of the limitations of the polymerase chain reaction (PCR) is the co-amplification of non-specific products caused by TaqPol activity
Enhanced gene targeting of adult and pluripotent stem cells using evolved adeno-associated virus
Gene correction, 169-179 (2014)
Measuring and monitoring apoptosis and drug toxicity in HIV patients by ligation-mediated polymerase chain reaction
Hooker DJ, et al.
Journal of Cellular and Molecular Medicine, 13(5), 948-958 (2009)
Application of real-time and multiplex polymerase chain reaction assays to study leaf blotch epidemics in barley
Fountaine JM, et al.
Phytopathology, 97(3), 297-303 (2007)
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