SK-N-F1

94092304, human nerve (bone marrow metastasis), Epithelial-like

• Code UNSPSC : 41106514
• Source biologique: human nerve (bone marrow metastasis)
• Mode de croissance: Adherent/aggregates in suspension
• Caryotype: Aneuploid
• Morphologie: Epithelial-like
• Produits: Not specified
• Récepteurs: Transferrin receptor
• Maladie(s) pertinente(s): metastasis

Propriétés

• Nom du produit: SK-N-F1, 94092304
• Source biologique: human nerve (bone marrow metastasis)
• Mode de croissance: Adherent/aggregates in suspension
• Caryotype: Aneuploid
• Morphologie: Epithelial-like
• Produits: Not specified
• Récepteurs: Transferrin receptor
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): metastasis
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human neuroblastoma

Description de la lignée cellulaire

SK-N-F1 has been obtained from a bone marrow metastasis of a male patient with neuroblastoma. The poorly differentiated and slow growing cells secrete different neuroblastoma specific peptides and synthesise choline acetyltransferase. Reactivity of anti-leukaemia-associated and anti-foetal brain-associated monoclonal antibodies could be shown. Epithelial-like and aggregate-forming populations.

Application

Carcinogenesis studies, cytotoxicity assays.

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,14
D16S539: 11,12
D5S818: 12,13
D7S820: 8,9
THO1: 6,9.3
TPOX: 8,9
vWA: 16,17

Milieu de culture

DMEM + 4mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/ml using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Clumped cells have tendency to float.

Autres remarques

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