B3795
Brain-derived neurotrophic factor human
BDNF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonyme(s) :
Abrineurin, BDNF
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About This Item
Source biologique
human
Niveau de qualité
Produit recombinant
expressed in E. coli
Pureté
≥95% (SDS-PAGE)
Forme
lyophilized powder
Qualité
endotoxin tested
Poids mol.
~27 kDa
Conditionnement
pkg of 10 μg
pkg of 5 μg
Conditions de stockage
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
Technique(s)
cell culture | mammalian: suitable
Impuretés
≤1.00 EU/μg Endotoxin level
Numéro d'accès UniProt
Température de stockage
−20°C
Description générale
BDNF is a member of the neurotrophin family of growth factors that includes NGF, NT-3, and NT-4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.1 BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non-neuronal cells.
The active form of BDNF recombinant human protein (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions
The active form of BDNF recombinant human protein (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions
Application
Brain-derived neurotrophic factor (BDNF) recombinant human protein has been used as a supplement in various types of culture media.
Actions biochimiques/physiologiques
Brain-derived neurotrophic factor (BDNF) is a 13.6 kDa (or 27.2 kDa dimer) member of the neurotrophin family. BDNF has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. BDNF is important in development and maintenance of neuronal populations within the central nervous system or cells directly associated with it. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.
Forme physique
lyophilized from a sterile 0.2 μm filtered solution with BSA
Remarque sur l'analyse
The biological activity is determined by its ability to stimulate the proliferation of human neuroblastoma cells, SH-SY5Y.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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Protocoles
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
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