H69

91091802, human lung, Not specified

• Synonyme(s) : H69 Cell Line
• Code UNSPSC : 41106514

Propriétés

• product name: H69, 91091802
• Source biologique: human lung
• Mode de croissance: Aggregates in suspension
• Caryotype: Aneuploid
• Morphologie: Not specified
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): metastasis
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian lung small cell carcinoma

Description de la lignée cellulaire

This cell line has been established from the pleural fluid of a 55 year old Caucasian male with small cell carcinoma of the lung prior to treatment. Inoculated into athymic mice, the cells form tumours with a typical histology of the original biopsy specimen. Elevated levels of the biomarkers L-dopa decarboxylase, bombesin-like immunoreactivity, neuron-specific enolase and brain isozyme of creatine kinase were reported. Cells grow as spherical floating aggregates.

Application

Tumourigenicity studies

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 11,13
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Maintain cultures between 6x10,000 to 3x100,000 cells/ml; 5% CO₂; 37°C.

Autres remarques

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