KARPAS-25

06072601

• Synonyme(s) : KARPAS 25, KARPAS, 25, KARPAS25
• Code UNSPSC : 41106514

Propriétés

• Source biologique: human lung (effusion)
• Mode de croissance: Suspension
• Caryotype: Hyperdiploid with complex aberrant chromosomes
• Morphologie: Round to polygonal cells growing singly in suspension
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice

Description

Origine de la lignée cellulaire

Human acute plasma cell leukaemia

Description de la lignée cellulaire

Established in 1989 from the pleural effusion of a 27-year old woman diagnosed with acute plasma cell leukaemia who failed to respond to treatment and died within 3 weeks.

Milieu de culture

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

Procédure de repiquage

Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 10⁵ cells/ml; 5% CO₂; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 10⁵ cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 10⁵ cells/ml, this tends to equate to a 1:3 split every 2-3 days.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Clause de non-responsabilité

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.