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Key Documents

CA201

Sigma-Aldrich

cAMP Enzyme Immunoassay Kit

sufficient for 96 assays

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 96 assays

Niveau de qualité

Technique(s)

ELISA: suitable

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... CAMP(820)

Description générale

Non-radioactive, competitive immunoassay for the quantitation of cAMP in biological fluids (serum, plasma, and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cAMP to competitively bind cAMP or cAMP which has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the additon of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cAMP present in the well.

Application

cAMP Enzyme Immunoassay Kit has been used to quantify the cAMP level.

Autres remarques

Please refer to the attached Protocolfor details.

Composants de kit seuls

Réf. du produit
Description

  • Acetic Anhydride 1 x 1

  • Assay Buffer 2 1 x 30

  • cAMP-Alkaline Phosphatase Conjugate 1 x 5

  • cAMP Assay Layout Sheet 1 x 1

  • cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5

  • 5 Cycle Logit-Log Paper 1 x 1

  • Cyclic AMP Standard 1 x 0.5

  • Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea

  • p-Nitrophenyl Phosphate Substrate Solution 1 x 20

  • Plate Sealer 1 ea

  • Stop Solution 1 x 5

  • Triethylamine 1 x 2

  • Wash Buffer Concentrate 1 x 30

Afficher tout (13)

Pictogrammes

FlameSkull and crossbonesCorrosion

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1A - STOT SE 3

Organes cibles

Respiratory system

Risques supp

Code de la classe de stockage

3 - Flammable liquids


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Cyclic Nucleotide Signaling and the Cardiovascular System, 148-148 (2018)
Lingyue Yuan et al.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 40(10), 1465-1471 (2020-10-30)
To elucidate the pathogenic role of leukotriene B4 (LTB4) in pulmonary hyper-permeability and inflammation induced by lung-protective mechanical ventilation (LPMV) in rabbits. Thirty-two healthy Japanese white rabbits were randomized into 4 groups for treatment with vehicle or bestatin (a leukotriene
Role of beta3-adrenoceptors for intrahepatic resistance and portal hypertension in liver cirrhosis
Trebicka J, et al.
Hepatology, 50(6), 1924-1935 (2009)
Monika Schmoll et al.
Eukaryotic cell, 8(3), 410-420 (2009-01-13)
Although the enzymes enabling Hypocrea jecorina (anamorph Trichoderma reesei) to degrade the insoluble substrate cellulose have been investigated in some detail, little is still known about the mechanism by which cellulose signals its presence to the fungus. In order to
The cyclic AMP-dependent catabolite repression system of Serratia marcescens mediates biofilm formation through regulation of type 1 fimbriae
Kalivoda EJ, et al.
Applied and Environmental Microbiology, 74(11), 3461-3470 (2008)

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