CGD1
Cell Growth Determination Kit, MTT based
Synonyme(s) :
Mitochondrial activity assay
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75
Produits recommandés
Conditionnement
pkg of 1 kit
Technique(s)
UV/Vis spectroscopy: suitable
protein quantification: suitable
Amax
570 nm
Application(s)
cell analysis
detection
Méthode de détection
colorimetric
Conditions d'expédition
dry ice
Température de stockage
−20°C
Description générale
Cell Growth Determination Kit (MTT based) is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) system is a simple, accurate, reproducible means of measuring the activity of living cells via mitochondrial dehydrogenase activity.
Application
Cell Growth Determination Kit, MTT based has been used:
- to measure the cell viability of preosteoblastic cells
- in MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of normal human prostatic stromal and epithelial cell lines
- to determine the cell viability of primary cervical epithelial cells
Conditionnement
Each kit includes 5 vials each containing 1 mL of 5 mg/ml MTT in RPMI (without phenol red) and 50 mL of MTT solvent (0.1 N HCl in anhydrous isopropanol). Volumes of kit components have been optimized for cultures grown in multi-well plates. MTT solutions is added at 10% of total culture volume, thus the number of tests per kit is dependent upon culture vessel and/or volume.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3
Organes cibles
Central nervous system
Code de la classe de stockage
3 - Flammable liquids
Point d'éclair (°F)
53.6 °F - closed cup
Point d'éclair (°C)
12 °C - closed cup
Certificats d'analyse (COA)
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