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C9284

Sigma-Aldrich

Catalase−Agarose

pH 5.4(isoelectric point), ammonium sulfate suspension, enzyme from bovine liver

Synonyme(s) :

H2O2:H2O2 Oxidoreductase

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About This Item

Numéro de classification (Commission des enzymes):
1.11.1.6 (BRENDA, IUBMB)
Numéro MDL:
MFCD00081483
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Source biologique

enzyme from bovine liver

Description

optimum pH ~ 7.0

Forme

ammonium sulfate suspension

Poids mol.

tetramer ~250 kDa

Concentration

≥800 units/mL

Ampleur du marquage

60,000-120,000 units per g agarose (1900-3800 units per ml of agarose)

Matrice

4% cross-linked beaded agarose

pH

5.4(isoelectric point)

Température de stockage

2-8°C

Application

Catalase agarose has been used in a study to advance the treatment of oxidative stress in neurodegenerative diseases. Catalase agarose has also been used in a study to investigate the growth of cyanobacterium on agar media in the presence of heterotrophic bacteria.
Catalases have a wide range of industrial applications. The enzyme is used for the removal of traces of (H2O2) that is added to milk and cheese during sterilization. It is used for the degradation of peroxide used in textile bleaching to prevent any problems in subsequent dyeing. Coupled with oxidases, the enzyme is used to prevent the inactivation of oxidases by the action of high concentration of peroxide. It is also used to prevent side reactions caused by (H2O2) that can degrade the target product.

Actions biochimiques/physiologiques

Catalase activity is constant over the pH range of 4.0-8.5. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule.

Attention

Freezing of solutions is not recommended.

Définition de l'unité

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

Forme physique

Suspension in 3.2 M (NH4)2SO4, pH 7, containing preservative

Inhibiteur

Réf. du produit
Description
Tarif

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Listes réglementaires

Les listes réglementaires sont principalement fournies pour les produits chimiques. Seules des informations limitées peuvent être fournies ici pour les produits non chimiques. L'absence d'indication signifie qu'aucun des composants n'est répertorié. Il incombe à l'utilisateur de s'assurer de l'utilisation sûre et légale du produit.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificats d'analyse (COA)

Lot/Batch Number
SLBH4018V
SLBB9121V
061M5171V
061M5171
070M5159

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Shohei Hayashi et al.
Microbes and environments, 26(2), 120-127 (2011-04-20)
The cyanobacterium Synechococcus leopoliensis CCAP1405/1 does not grow on common solid media made of agar, agarose HT, noble agar, gelrite and gelatin, although it grows in liquid media with the same components. The inoculation of S. leopoliensis CCAP1405/1 at a
Jing Yu et al.
Neurological research, 33(7), 708-716 (2011-07-16)
4-N-trimethylammonium-3-hydroxybutyric acid (L-carnitine) is an endogenous mitochondrial membrane compound and some studies have reported that L-carnitine could effectively protect various cells against oxidative injury both in vitro and in vivo. In the present study, we used the human neuroblastoma SH-SY5Y
Lorena Betancor et al.
Biotechnology progress, 19(3), 763-767 (2003-06-07)
Bovine liver catalase was immobilized on different supports. The tetrameric nature of this enzyme was found to cause its rapid inactivation in diluted conditions due to subunit dissociation, a fact that may rule out its industrial use. Multi-subunit immobilization using
Azizah Ugusman et al.
BMC complementary and alternative medicine, 11, 31-31 (2011-04-19)
Aqueous extract of Piper sarmentosum (AEPS) is known to possess antioxidant and anti-atherosclerotic activities but the mechanism responsible for it remains unclear. In early part of atherosclerosis, nuclear factor-kappa B (NF-κB) induces the expression of cellular adhesion molecules such as
Angela Sacchi et al.
Environmental pollution (Barking, Essex : 1987), 177, 82-89 (2013-03-12)
Coastal lagoons are constantly subjected to releases of chemical pollutants, and so organisms may be exposed to such toxicants. This study investigated through a multivariate approach the physiological status of bivalve Ruditapes philippinarum, farmed in Sacca di Goro lagoon. Biomarkers
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