A3422

Achromopeptidase from Lysobacter enzymogenes

partially purified powder, ≥20,000 units/mg solid

• Synonyme(s) : ACP, Achromopeptidase
• Numéro de classification (Commission des enzymes): 3.4.21.50 (BRENDA, IUBMB)
• Numéro MDL: MFCD00130333
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Forme: partially purified powder
• Niveau de qualité: 200
• Activité spécifique: ≥20,000 units/mg solid
• Poids mol.: 27 kDa
• Activité étrangère: Collagenase, present
• Température de stockage: −20°C

Description

Application

Achromopeptidase from bacteria may be used:

• in the homogenization step for genomic DNA extraction from microbial biofilm
• in the lysis of gram-positive strains(
• for DNA extraction from Nocardia strains

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Product A3422 is from bacteria and is a partially purified powder containing lactose.

Actions biochimiques/physiologiques

Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀ = 0.6 over 2 hours at 37 °C.

Définition de l'unité

One unit will produce a change in A₆₀₀ of 0.001 per minute per mL at pH 8.0 at 37 °C using a suspension of Micrococcus lysodeikticus as substrate (1 cm light path).

Forme physique

Partially purified powder.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P342 + P311
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Faceshields, Gloves