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A3076

Sigma-Aldrich

Anti-Human IgE−Alkaline Phosphatase antibody, Mouse monoclonal

clone GE-1, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-Human IgE, Monoclonal Anti-Human IgE−Alkaline Phosphatase antibody produced in mouse

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

mouse

Conjugué

alkaline phosphatase conjugate

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

secondary antibodies

Clone

GE-1, monoclonal

Forme

buffered aqueous solution

Espèces réactives

human

Technique(s)

direct ELISA: 1:6,000
dot blot: 1:1,000

Isotype

IgG2b

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

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Catégories apparentées

Description générale

Binds human IgE; does not bind human IgG, IgA or IgM.
IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma.
Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the GE-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Mouse monoclonal anti-human IgE-alkaline phosphatase antibody has been used for :
  • immunoblot assays
  • direct ELISA
  • dot blot
To confirm that a chimaeric monoclonal antibody had episolon chain, an elisa was performed using monoclonal anti-human IgE antibody (clone GE1) at a 1:1000 dilution.

Actions biochimiques/physiologiques

IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma.

Forme physique

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

Remarque sur l'analyse

The antibody is tested for reactivity against myeloma IgE and allergy-elevated IgE.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Kyoung Yong Jeong et al.
Clinical and diagnostic laboratory immunology, 11(2), 320-324 (2004-03-12)
Chironomids are widely and abundantly distributed in the vicinity of standing waters. Larvae of Chironomus and some other genera are known to contain hemoglobins, which have been described as a major allergen, and the adults that have no hemoglobins also
Nathalie Acevedo et al.
PloS one, 8(11), e78353-e78353 (2013-11-14)
Helminth infections and allergy have evolutionary and clinical links. Infection with the nematode Ascaris lumbricoides induces IgE against several molecules including invertebrate pan-allergens. These antibodies influence the pathogenesis and diagnosis of allergy; therefore, studying parasitic and non-parasitic allergens is essential
A Diaz-Perales et al.
The Journal of allergy and clinical immunology, 102(1), 127-133 (1998-07-29)
Several foods associated with the latex-fruit syndrome present relevant allergens of around 30 kd. Neither these components nor any other responsible for the reported cross-reactions have been identified and purified. We sought to isolate and characterize the 30 kd allergens
Astrid R R Heutelbeck et al.
International archives of occupational and environmental health, 82(9), 1123-1131 (2009-02-25)
Cattle are an important source of allergens in the working area of farmers. Asthma caused by cow allergens is a significant occupational problem. Yet in allergological testing, the results of in vivo and in vitro diagnostic tests are often inconsistent
Marta Burzyńska et al.
Acta scientiarum polonorum. Technologia alimentaria, 19(1), 15-24 (2020-04-01)
Small amounts of protein can be found in honey, including well known allergen sources, such as plant pollen and honeybee secretions. Despite this, there are few case reports describing allergic reactions following the consumption of honey. The aim of this
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