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Key Documents

A0293

Sigma-Aldrich

Anti-Human IgG (Fab specific)−Peroxidase antibody produced in goat

affinity isolated antibody

Synonyme(s) :

Anti Human Fab Antibody, Anti Human Fab Antibody - Anti-Human IgG (Fab specific)-Peroxidase antibody produced in goat

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About This Item

Numéro MDL:
MFCD00162434
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

goat

Niveau de qualité

300

Conjugué

peroxidase conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Espèces réactives

human

Technique(s)

direct ELISA: 1:40,000
dot blot: 1:100,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot (chemiluminescent): 1:100,000

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders,.
Antibody is isolated from anti-human IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. Anti-Human IgG is conjugated to peroxidase and then further purified to remove unconjugated material.

Immunogène

Fab fragment of human IgG

Application

Anti-Human IgG (Fab specific)-Peroxidase antibody produced in goat has been used in:
  • western blot
  • direct enzyme linked immunosorbent assay (ELISA) at 1:40,000 dilution for analysis of yeast cultures genetically modified to produced recombinant Fab fragments.
  • immunohistochemistry
  • dot blot
  • immunohistochemistry (formalin-fixed, paraffin-embedded sections, 1:200)

Anti-Human IgG antibody was used at a 1:10,000 dilution in PBS and incubated for 1 hour at room temperature. Binding in ELISA assays was detected with a O-phenylene-diamine substrate solution (Sigma).
Goat anti-human IgG (Fab specific)-peroxidase antibody can be used for direct ELISA (1:40,000), dot blot and immunohistochemistry (formalin-fixed, paraffin-embedded sections, 1:200) applications.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Notes préparatoires

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

An immuno-assay to quantify influenza virus hemagglutinin with correctly folded stalk domains in vaccine preparations
Rajendran M, et al.
PLoS ONE, 13(4), e0194830-e0194830 (2018)
Metabolic responses of CHO cells to limitation of key amino acids
Duarte TM, et al.
Biotechnology and Bioengineering, 111(10), 2095-2106 (2014)
James Duehr et al.
mSphere, 3(1) (2018-02-13)
Recent reports in the scientific literature have suggested that anti-dengue virus (DENV) and anti-West Nile virus (WNV) immunity exacerbates Zika virus (ZIKV) pathogenesis in vitro and in vivo in mouse models. Large populations of immune individuals exist for a related
A novel approach of prophylaxis to HBV recurrence after liver transplantation
Pan T, et al.
Virology, 382(1), 1-9 (2008)
Daniel Stadlbauer et al.
mSphere, 2(6) (2017-12-16)
Human influenza virus infections with avian subtype H7N9 viruses are a major public health concern and have encouraged the development of effective H7 prepandemic vaccines. In this study, baseline and postvaccination serum samples of individuals aged 18 years and older
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