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Principaux documents

A1595

Sigma-Aldrich

Albumine de sérum bovin solution

10% in DPBS, low endotoxin, fatty acid free, suitable for cell culture, sterile-filtered

Synonyme(s) :

Albumine solution from bovine serum, Solution de BSA

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About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.27

Source biologique

bovine

Niveau de qualité

Stérilité

sterile-filtered

Gamme de produits

BioReagent

Essai

90-120 mg/mL protein basis (biuret)

Forme

solution

Produit purifié par

heat shock fractionation

Contient

does not contain stabilizer or preservative

Origine

USA origin

Concentration

10% in DPBS

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤0.05% fatty acid
≤200 EU/mL endotoxin

pH

7.1-7.7

Numéro d'accès UniProt

Température de stockage

2-8°C

Informations sur le gène

bovine ... ALB(280717)

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Application

Recommended for use as a base for the preparation of defined, fatty acid supplements. Can be loaded with up to 4 moles of fatty acids per mole of albumin.

BSA is a known fatty acid (FA) carrier. Thus in FA-related applications like cell culture, the specific BSA can be important, as related to control over specific fatty acids used to culture cell lines, because different cell lines can be variably sensitive to particular FA;s. Fatty acid-free BSA therefore is useful in cell culture work where the specific fatty acid content must be carefully controlled, so that researchers can control the specific FA′s needed for their particular cell lines. Fatty acid-free BSA also allows for optimal and maximum binding sites for the specific fatty acids that researchers wish to use in their particular cell culture experiments. Use of fatty-acid-free BSA also addresses concerns about endogenous FA′s potentially in non-fatty acid-free BSA.

Caractéristiques et avantages

  • Fatty acid free / tested to be low in fatty acid content
  • Low endotoxin
  • Sterile-filtered

Notes préparatoires

Prepared with Heat Shock Fractionated fatty acid-free albumin in DPBS D5652.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

Ana Janic et al.
Nature medicine, 24(7), 947-953 (2018-06-13)
It has long been assumed that p53 suppresses tumor development through induction of apoptosis, possibly with contributions by cell cycle arrest and cell senescence1,2. However, combined deficiency in these three processes does not result in spontaneous tumor formation as observed
Rosa G M Lammerts et al.
American journal of physiology. Renal physiology, 320(5), F947-F962 (2021-03-16)
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Lingjun Zhang et al.
Neural plasticity, 2022, 5567174-5567174 (2022-02-01)
Sensorineural hearing loss (SNHL) is one of the most common causes of disability worldwide. Previous evidence suggests that reactive oxygen species (ROS) may play an important role in the occurrence and development of SNHL, while its mechanism remains unclear. We
Jingang Hou et al.
Journal of ginseng research, 46(1), 79-90 (2022-01-22)
Herbal medicines are popular approaches to capably prevent and treat obesity and its related diseases. Excessive exposure to dietary lipids causes oxidative stress and inflammation, which possibly induces cellular senescence and contribute the damaging effects in brain. The potential roles
Surasak Kasetsirikul et al.
The Analyst, 145(23), 7680-7686 (2020-09-26)
This work reports the development of a rapid, simple and inexpensive colorimetric paper-based assay for the detection of the severe acute respiratory symptom coronavirus 2 (SARS-CoV-2) humanized antibody. The paper device was prepared with lamination for easy sample handling and

Articles

This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

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