83264

HEPES solution

1 M in H₂O, liquid

• Synonyme(s) : Acide N-(2-hydroxyéthyl)pipérazine-N′-(2-éthanesulfonique)
• Numéro CAS: 7365-45-9
• Numéro MDL: MFCD00006158
• Code UNSPSC : 12161700
• eCl@ss : 32129211
• ID de substance PubChem : 57652951
• Nomenclature NACRES : NA.25

Propriétés

• product name: HEPES solution, 1 M in H₂O
• Forme: liquid
• Concentration: 1 M in H₂O; 238 g/L HEPES in H₂O
• Impuretés: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH: 5.5±0.5
• Plage de pH utile: 6.8-8.2
• Densité: 1.07 g/mL at 20 °C
• Application(s): diagnostic assay manufacturing
• Chaîne SMILES: OCCN1CCN(CCS(=O)(O)=O)CC1
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• Clé InChI: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

Description générale

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:

• As a component of SAPI medium used for the culturing of S. Typhymurium inoculum
• To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI
• As a component of dissection buffer for culturing rat cortical neurons
• Along with MgCl₂, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses
• As a component of Danieu′s aquarium water used in agarose gel preparation

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)