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Key Documents

125-50

SAFC

Collagen Coating Solution (50 ML)

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Niveau de qualité

100

Forme

liquid

Durée de conservation

1 yr

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

Collagen is a major fibrous, structural protein in animals. It is a key component of the extracellular matrix (ECM) and is predominantly present in the connective tissue, ligaments, tendons, and cornea. Collagens consist of three parallel left-handed polypeptide chains coiled to form a right-handed triple helix. They also have a globular domain.

Application

The coating concentration is 1 ml per 10 cm2 surface area of the cell culture ware: 2.5 ml for coating a T-25 flask or a 60 mm tissue culture dish. 7.5 ml for coating a T-75 flask or a 100 mm tissue culture dish.

Add Attachment Factor Solution to the flask and rock the flask gently to distribute the solution evenly to cover the whole culture surface. Coat the culture ware surface for 30 minutes at 37°C or 2 hours or overnight at room temperature. Remove Attachment Factor Solution by aspiration in a sterile hood. The coated flask can be used immediately or store at 4°C for up to 2 weeks.

Actions biochimiques/physiologiques

Collagen is a basic unit for the growth and support of various cells and tissues in the body. Collagen may be involved in wound healing. Collagen might promote cell adhesion and proliferation when used as a coating on iron-manganese alloys (Fe-30%Mn) scaffolds in orthopedic applications.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Henry Bautista-Amorocho et al.
BMC molecular and cell biology, 22(1), 1-1 (2021-01-08)
Culturing primary epithelial cells has a major advantage over tumor-derived or immortalized cell lines as long as their functional phenotype and genetic makeup are mainly maintained. The swine model has shown to be helpful and reliable when used as a
Rajini Nagaraj et al.
Nanomaterials (Basel, Switzerland), 10(3) (2020-04-03)
Diabetic retinopathy (DR), Retinopathy of Pre-maturity (ROP), and Age-related Macular Degeneration (AMD) are multifactorial manifestations associated with abnormal growth of blood vessels in the retina. These three diseases account for 5% of the total blindness and vision impairment in the

Protocoles

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