10112001

COCA cell line from mouse

• Synonyme(s) : COCA cells
• Code UNSPSC : 41106514
• Nomenclature NACRES : NA.81

Propriétés

• Source biologique: mouse skin
• Mode de croissance: Adherent
• Caryotype: See Segrelles et al 2011
• Morphologie: Epithelial
• Produits: See Segrelles et al 2011
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse epidermal keratinocyes, differentiation

Description de la lignée cellulaire

COCA, a murine epidermal cell line was developed by serially passaging keratinocytes from the back skin of adult C57BL/DBA mice. Culture of the COCA cells is in fully defined media without requirement for feeder layer or other coating. COCA retains its ability to differentiate and stratify in response to increased calcium concentrations thus providing an excellent experimental system for in vitro (conventional and 3D epidermal cell cultures) and in vivo (skin regeneration) skin modelling.

Application

For complete product information, please see ECACC

Milieu de culture

CnT-07 media This defined medium contains low calcium (0.07mM). To promote good cell attachment post trypsinisation, an 8-14 hours incubation in CnT-07 medium containing 0.2mM CaCl2 is necessary. Differentiation can be initiated by transferring a confluent monolayer from 0.07mM calcium to high calcium (1.2mM).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 0.5-1.5 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor. To remov

Autres remarques

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Cultures from HPA Culture Collections, supplied by us, are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3