PPB022

Tris buffer

Pre-set, pH 7.0, pHast Pack^™, powder

• Synonyme(s) : 0.1M Tris
• Code UNSPSC : 12161700
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 100
• Gamme de produits: pHast Pack^™
• Forme: powder
• Conditions de stockage: (Tightly closed. Dry)
• Impuretés: ≤5 ppm Heavy Metals (lead)
• Couleur: white
• pH: 7.0
• Solubilité: soluble, clear, colorless
• Traces de cations: Fe: ≤10 ppm
• Activité étrangère: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• Température de stockage: room temp

Description

Description générale

Tris buffer, pH 7.0 is a general buffer that is isotonic and non-toxic in nature due to the presence of tris base. It is suitable for most biochemical and molecular biology applications. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.

Application

Tris buffer is used in:

• Western blot
• Protein gel electrophoresis
• cell culture (sterile filter buffer)
• As a buffer component

Caractéristiques et avantages

• Fast, ready-to-use buffer
• No need to measure and adjust the pH
• Saves time and minimizes effort in the lab
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Conditionnement

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 7.0 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.

Stockage et stabilité

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Autres remarques

For additional information on our range of Biochemicals, please complete this form.

Informations légales

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1