648315

Tris(hydroxyméthyl)aminométhane

100 mM liquid, pH 7.4, Molecular Biology Grade

• Synonyme(s) : 2-Amino-2-(hydroxyméthyl)propane-1,3-diol, Base Tris, THAM, Trométamol
• Formule linéaire : NH₂C(CH₂OH)₃
• Numéro CAS: 77-86-1
• Poids moléculaire : 121.14
• Numéro Beilstein : 741883
• Numéro MDL: MFCD00004679
• Code UNSPSC : 12161705

Propriétés

• product name: Tris(hydroxyméthyl)aminométhane, Widely used biological buffer.
• Qualité: Molecular Biology
• Niveau de qualité: 100
• Forme: liquid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Concentration: 100 mM
• Couleur: clear colorless
• Plage de pH utile: 7-9
• pKa (25 °C): 8.1
• Point d'ébullition: 219-220 °C/10 mmHg (lit.)
• Pf: 167-172 °C (lit.)
• Activité étrangère: DNases, RNases, none detected
• Conditions d'expédition: ambient
• Température de stockage: 10-30°C
• Chaîne SMILES: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• Clé InChI: LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

Description générale

Widely used biological buffer. Prepared with diethylpyrocarbonate-treated water for molecular biology applications. Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Widely used biological buffer. Suitable for DNA and RNA applications. Useful in the pH range of 7.0-9.0.

Application

• Structural Characterization of Toxicologically Relevant Cd(2+)-L-Cysteine Complexes.: This study utilized Tris buffer, 100 mM, pH 7.4, for the structural analysis of cadmium-cysteine complexes, providing insights into their biochemical behavior and toxicological impacts (Gautam et al., 2023).


• Cation and buffer specific effects on the DNA-lipid interaction.: The research employed Tris buffer, 100 mM, pH 7.4, to explore the specific effects of cations and buffers on DNA-lipid interactions, highlighting its importance in biophysical and biochemical studies (Mura et al., 2023).


• Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applications.: The research utilized Tris buffer, 100 mM, pH 7.4, in the preparation of cerium-containing nanoparticles, demonstrating their potential in biomedical applications without the need for drugs (Kurtuldu et al., 2021).

Avertissement

Toxicity: Irritant (B)

Forme physique

A sterile, 100 mM solution, pH 7.4.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable