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F5415

Sigma-Aldrich

Ficoll® solution

Type 400, 20% in H2O

Synonyme(s) :

Ready-to-use density gradient media

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About This Item

Numéro CAS:
26873-85-8
Numéro MDL:
MFCD00081599
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Stérilité

0.2 μm filtered

Niveau de qualité

200

Type

Type 400

Forme

liquid

Concentration

20% in H2O

Couleur

clear colorless

Solubilité

water: soluble

Densité

1.055-1.075 g/mL

Application(s)

hematology
histology

Température de stockage

2-8°C

Catégories apparentées

Description générale

Ficoll® Solution is a highly branched polymer of sucrose, also known as polydisperse mixtures of dextran. Ficoll® molecules are inert and not re-absorbed by proximal tubules. The structure is open and deformable, and not a rigid sphere thereby becoming a preferred choice in “crowding” experiments.
Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients.

Application

Ficoll® has been used in


  • Isolation of cord blood mononuclear cells
  • Isolation of CD133 negative cells
  • Isolation and expansion of circulating endothelial progenitor cells.

Autres remarques

A nonionic synthetic polymer of sucrose.

Informations légales

Ficoll is a registered trademark of Cytiva

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
SLCL2553
SLCJ9378
SLCH6817
SLCH3511
SLCH0230

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Xiaoliang Wu et al.
Shock (Augusta, Ga.), 51(6), 718-724 (2018-08-07)
The aim of this study was to investigate the correlation between plasma sphingosine-1-phosphate (S1P) and ceramide concentrations in sepsis, and the possible mechanisms for altered expression. Plasma S1P and ceramide concentrations were measured by HPLC-ESI-MS/MS. HLA-DR (human leukocyte antigen-DR) expression
Circulation enrichment of functional endothelial progenitor cells by infantile phototherapy.
Siavashi V, et al.
Journal of Cellular Biochemistry, 118(2), 330-340 (2017)
Juliane Schmidt et al.
PloS one, 6(3), e18184-e18184 (2011-03-30)
The characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have
Effect of mononuclear cells on morphometric of kidney parameters in hyperglycemic rat.
Salmani F, et al.
Annals of Experimental Biology, 2(3), 1-7 (2014)
Analysis of Structural and Functional Glomerular Changes in Hyperglycemic Rats that Treated with Human Umbilical Cord Blood Derived CD133 Negative Ccells.
Feizi F, et al.
Physical Chemistry, 1(1) (2014)
Afficher tous les articles scientifiques apparentés

Protocoles

Hematopoietic Stem Cell Culture Protocols

Step-by-step hematopoietic stem cell culture protocols for isolation, expansion and differentiation of CD34+ hematopoietic progenitor cells including CFU assays. It can be cultured under defined conditions designed either to promote self-renewal and increase the number of primitive cells.

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