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GE17-5446-52

Ficoll®-Paque PREMIUM 1.073

Cytiva 17-5446-52, pack of 6 × 100 mL

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About This Item

Code UNSPSC :
12352201
Nomenclature NACRES :
NA.25

Forme

liquid

Conditionnement

pack of 6 × 100 mL

Fabricant/nom de marque

Cytiva 17-5446-52

Couleur

Colorless to slightly yellow

Plage de pH

5.5-7.5

Description générale

Ficoll-Paque PREMIUM products are a range of sterile, ready-to-use density gradient media for the preparation of mononuclear cells for use in cell therapy applications.

Application

Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS, which has been used for more than 30 years as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2003.

Caractéristiques et avantages

  • Sterile, ready to use reagents used to prepare mononuclear cell preparations from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.
  • Manufactured under a Quality Management System certified to ISO 13485:2003.
  • Low levels of endotoxin (< 0.12 EU/mL) secured and tested.
  • Cytotoxicity grade 0.
  • A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Ficoll is a registered trademark of Cytiva

Code de la classe de stockage

12 - Non Combustible Liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Properties of Mononuclear Cells Isolated by the Ficoll-Paque Method

The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.

How to Fractionate and Analyze Gradients of Percoll

Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and will not work with other media. Using Density Marker Beads as an external marker facilitates monitoring of the gradient shape and range.

Removal of Percoll after Centrifugation

This page shows methods to remove Percoll from a sample after centrifugation.

Applications of Percoll in Other Cell Types

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

Protocoles

How to make and use gradients of Percoll

How to use percoll to prepare a gradient

Recommended Standard Method for Isolating Mononuclear Cells

The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

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