C4916

5-Carboxyfluorescein diacetate

~95% purity (HPLC), powder

• Synonyme(s) : 5-CFDA, 5-Carboxy-di-O-acetylfluorescein
• Formule empirique (notation de Hill): C₂₅H₁₆O₉
• Numéro CAS: 79955-27-4
• Poids moléculaire : 460.39
• Numéro Beilstein : 68265
• Numéro MDL: MFCD00036872
• Code UNSPSC : 12171500
• ID de substance PubChem : 24892738
• Nomenclature NACRES : NA.47

Propriétés

• product name: 5-Carboxyfluorescein diacetate, ~95% (HPLC)
• Pureté: ~95% (HPLC)
• Niveau de qualité: 200
• Forme: powder
• Solubilité: chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: −20°C
• Chaîne SMILES: CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(O)=O)c1
• InChI: 1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)
• Clé InChI: WPUZGNPQMIWOHE-UHFFFAOYSA-N

Description

Application

5-Carboxyfluorescein diacetate has been used for the staining of bacteria to study bacterial adhesion to mucin.

Actions biochimiques/physiologiques

5-Carboxyfluorescein diacetate is required for the staining of cells to differentiate between live and dead cells. It helps in monitoring integrity and intactness of cell membrane. 5-Carboxyfluorescein diacetate is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product carboxyfluorescein. In viable cells, the cellular membrane is impermeable to the fluorescent molecule.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges