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114413

Sigma-Aldrich

Bromothymol Blue

ACS reagent, Dye content 95 %

Synonyme(s) :

3′,3″-Dibromothymolsulfonphthalein

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About This Item

Formule empirique (notation de Hill):
C27H28Br2O5S
Numéro CAS:
76-59-5
Poids moléculaire :
624.38
Numéro Beilstein :
373934
Numéro CE :
200-971-2
Numéro MDL:
MFCD00005872
Code UNSPSC :
12171500
ID de substance PubChem :
24847171
Nomenclature NACRES :
NA.47

Qualité

ACS reagent

Niveau de qualité

200

Forme

powder

Composition

Dye content, 95%

Couleur

pink-purple

visual transition interval

6.0-7.6, yellow to blue(passes test)

Pf

200-202 °C ((392 - 396 °F) - lit.)
200-202 °C (lit.)

λmax

420 nm

ε (coefficient d'extinction)

≥17000 at 419-425 nm in methanol
≥8000 at 326-332 nm in methanol
≥9000 at 279-285 nm in methanol

Température de stockage

room temp

Chaîne SMILES 

CC(C)c1cc(c(C)c(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C(C)C)c(O)c(Br)c4C

InChI

1S/C27H28Br2O5S/c1-13(2)17-11-20(15(5)23(28)25(17)30)27(19-9-7-8-10-22(19)35(32,33)34-27)21-12-18(14(3)4)26(31)24(29)16(21)6/h7-14,30-31H,1-6H3

Clé InChI

NUHCTOLBWMJMLX-UHFFFAOYSA-N

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Description générale

Bromothymol Blue, also known as, dibromothymolsulfonphthalein is a sulfonated hydroxyquinone in acidic aqueous solutions. In alkaline solutions, this dye is present as a moderately sized dianion. It is also known as bromthymol blue and dibromothymolsulfonphthalein. It is a pH-sensitive dye that changes color from yellow to blue.

Application

Bromothymol blue dye serves as a pH indicator because of the precise color transition at neutrality. Other applications include:
  • as a vital stain to trace the movement of fluids from the lymph and to define cell walls or nuclei under the microscope
  • for the demonstration of fungal hyphae within plant roots
  • in the assessment of the soluble acidity within single rice grains
  • use in an agar gel medium for enumeration of Bacillus cereus
  • as a component of several bacterial growth and detection substrates such as cystine lactose electrolyte deficient (CLED/BROLACIN); polymyxin pyruvate egg yolk mannitol bromothymol blue (PEMBA) and MacConkey agar.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
SHBN5829
MKCK0744
MKCG9344
MKCG4045
MKCC4196

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A pH colorimetric sensor array (CSA) with fast response time (<1 min) using only one acid-base indicator, Bromothymol Blue (BB), was prepared and characterized by modulating the amount, C, of the surfactant Hexadecyltrimethylammonium p-toluenesulfonate between 0 and 0.3725 gCTApTs/gprecursor with a
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