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114375

Sigma-Aldrich

Bromocresol Purple

BioReagent, suitable for indicator, Dye content 90 %

Synonyme(s) :

5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form

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About This Item

Formule empirique (notation de Hill):
C21H16Br2O5S
Numéro CAS:
Poids moléculaire :
540.22
Numéro Beilstein :
359618
Numéro CE :
Numéro MDL:
Code UNSPSC :
12171500
ID de substance PubChem :
Nomenclature NACRES :
NA.47

Gamme de produits

BioReagent

Niveau de qualité

Pureté

≥89.0% (HPLC)

Forme

powder

Composition

Dye content, 90%

Technique(s)

titration: suitable

pH

5.2-6.8, yellow to purple

visual transition interval

5.2-6.8, yellow to blue

Pf

240 °C (dec.) (lit.)

Solubilité

ethanol: 1 mg/mL

λmax

419 nm

ε (coefficient d'extinction)

≥22000 at 418-424 nm in methanol

Adéquation

suitable for indicator

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4

InChI

1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3

Clé InChI

ABIUHPWEYMSGSR-UHFFFAOYSA-N

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Application

Bromocresol Purple has been used for staining of Saccharomyces cerevisiae.
Spectrophotometric determination of serum-albumin

Actions biochimiques/physiologiques

Bromocresol purple is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves


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Consulter la Bibliothèque de documents

Yeast colonies synchronise their growth and development.
Palkova Z and Forstova J
Journal of Cell Science, 113, 1923-1928 (2000)
ATP leakage from yeast cells treated by extracellular glycolipids of Pseudozyma fusiformata.
Kulakovskaya TV, et al.
FEMS Yeast Research, 3, 401-404 (2003)
Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity.
Kurzweilova H and Sigler K
Yeast, 9, 1207-1211 (1993)
Munzer Kattan et al.
Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine, 69(2), 377-380 (2010-11-27)
Polyvinyl chloride (PVC) dyed with bromocresol purple was investigated as a high-dose radiation dosimeter. The absorbance at 417 nm depends linearly on the dose below 50 kGy. The response depends neither on dose rate nor on the irradiation temperature. The
Faizah Mohd Faizul et al.
Journal of photochemistry and photobiology. B, Biology, 90(1), 1-7 (2007-11-21)
The interaction between bromocresol purple (BCP) and bovine serum albumin (BSA)/porcine serum albumin (PSA) was investigated both in the absence and presence of bilirubin (BR) using absorption/absorption difference spectroscopy. A significant red shift in the absorption maxima of BCP accompanied

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