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Merck
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11214667001

Roche

Anti-Digoxigenin, Fab fragments

from sheep

Synonyme(s) :

anti-digoxigenin, digoxigenin

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About This Item

Code UNSPSC :
12352203

Source biologique

sheep

Niveau de qualité

100

Conjugué

unconjugated

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

lyophilized

Conditionnement

pkg of 1 mg

Fabricant/nom de marque

Roche

Technique(s)

ELISA: 2-4 μg/mL
immunohistochemistry: 0.5-1 μg/mL
western blot: 0.5-2 μg/mL

Isotype

IgG1

Température de stockage

2-8°C

Description générale

Anti-Digoxigenin, Fab fragments are Fab fragments from polyclonal anti-digoxigenin antibodies. Anti-Digoxigenin Fab fragments are preferentially used for secondary detection using a light chain specific anti-sheep conjugate.
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems.

Spécificité

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

Application

Anti-Digoxigenin Fab fragments are preferentially used for secondary detection using a light chain specific anti-sheep conjugate.
Use Anti-Digoxigenin, Fab fragments for the detection of digoxigenin-labeled compounds using:
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Western blot
  • Flow cell construction.

Caractéristiques et avantages

Specific to digoxigenin and digoxin.

Séquence

The number of amino groups in the Fab fragments is not known, because polyclonal antibodies are not sequenced. But as a rule of thumb full length antibodies contain about 80 amino groups on average.

Notes préparatoires

Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: 2 to 4 μg/ml
  • Immunohistocytochemistry: 0.5 to 1 μg/ml
  • In situ hybridization: 0.2 to 0.4 μg/ml
  • Western blot: 0.5 to 2 μg/ml

Storage conditions (working solution): Diluted antibody is stable at 2 to 8 °C for 12 hours.
Always prepare freshly!

Reconstitution

Add 1 ml PBS to a final concentration of 1 mg/ml. Centrifuge reconstituted antibody for 5 minutes at 10,000 rpm prior to each use, and pipet the necessary amount carefully from the surface.

Remarque sur l'analyse

Preparation: After immunization with digoxigenin, sheep IgGs are purified by ion exchange chromatography. Specific IgGs are isolated by immunosorption. Fab fragments obtained after papain digestion are purified by gel filtration and lyophilized.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

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Bompani R, et al.
Giornale di gerontologia, 18(8), 720-734 (1970)
Véronique Van De Bor et al.
Development (Cambridge, England), 148(4) (2021-02-03)
Basement membranes (BM) are extracellular matrices assembled into complex and highly organized networks essential for organ morphogenesis and function. However, little is known about the tissue origin of BM components and their dynamics in vivo Here, we unravel the assembly
Annealing helicase HARP closes RPA-stabilized DNA bubbles non-processively
Burnham DR, et al.
Nucleic Acids Research, 45(8), 4687-4695 (2017)
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Katano A, et al.
Bioimages, 25, 1-6 (2017)
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