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PMEF-NX

Sigma-Aldrich

EmbryoMax® Primary Mouse Embryonic Fibroblasts

Mouse

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About This Item

Code UNSPSC :
41106509
eCl@ss :
32011203
Nomenclature NACRES :
NA.75

product name

EmbryoMax® Primary Mouse Embryonic Fibroblasts, PMEF, Neo Resistant, Strain FVB, Irradiated, Passage 3

Source biologique

mouse

Niveau de qualité

100

Fabricant/nom de marque

Specialty Media
EmbryoMax®

Technique(s)

cell culture | stem cell: suitable

Entrée

sample type: mouse embryonic stem cell(s)
sample type primary embryotic fibroblasts (PMEFs)
sample type induced pluripotent stem cell(s)

Conditions d'expédition

liquid nitrogen

Description générale

Primary Mouse Embryo Fibroblasts, neo resistant, Strain FVB, are mitotically inactivated by treatment with irradiation and serve as a feeder layer for both mouse and human ES/IPS cells. MEF cells are resistant to neomycin.

Plating MEF Feeder Cells

Procedure:

1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
appearance. Gelatinized plates may be used for 12–14 days.
The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

Description de la lignée cellulaire

Primary Mouse Embryo Fibroblasts

Application

Research Category
Stem Cell Research

Conditionnement

5-6x106 ea

Stockage et stabilité

Vials should be stored in the vapor phase of liquid nitrogen.

Informations légales

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Protocoles

Induced Pluripotent Stem Cell Reprogramming Protocols

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

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