MABN2440

Anti-ABCA4 Antibody, clone 5B4

clone 5B4, from mouse

• Synonyme(s) : Retinal-specific ATP-binding cassette transporter, ATP-binding cassette sub-family A member 4, RIM ABC transporter, RIM protein, RmP, Stargardt disease protein
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 5B4, monoclonal
• Espèces réactives: bovine, human
• Réactivité de l'espèce (prédite par homologie): mouse (based on 100% sequence homology)
• Conditionnement: antibody small pack of 25 μg
• Technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG2bκ
• Numéro d'accès NCBI: NP_000341.2
• Numéro d'accès UniProt: P78363
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ABCA4(24)

Description

Description générale

Retinal-specific ATP-binding cassette transporter (UniProt: P78363; also known as ATP-binding cassette sub-family A member 4, ABCA4, RIM ABC transporter, RIM protein, RmP, Stargardt disease protein) is encoded by the ABCA4 (also known as ABCR) gene (Gene ID: 24) in human. ABCA4 is a multi-pass membrane protein that is exclusively expressed in retina and is found in the rims of rod photoreceptor cells. It contains two ABC transporter domains (aa 929-1160 and 1938-2170). In the visual cycle, it act as a retinal extruder or retinal-phosphatidylethanolamine flippase to facilitate the removal of all-trans-retinal from disc membranes following the photobleaching of rhodopsin. Mutations in ABCA4 gene are reported to cause Stargardt disease, which is characterized by decreased central vision, atrophy of the macula and underlying retinal pigment epithelium, and frequent presence of prominent flecks in the posterior pole of the retina. It has also been linked to age-related macular degeneration and retinitis pigmentosa.

Spécificité

Clone 5B4 detects Retinal-specific ATP-binding cassette transporter protein in human and bovine. It targets an epitope within 249 amino acids from the N-terminal half.

Immunogène

GST-tagged recombinant fragment corresponding to 249 amino acids from the N-terminal half of human Retinal-specific ATP-binding cassette transporter.

Application

Anti-ABCA4, clone 5B4, Cat. No. MABN2440, is a highly specific mouse monoclonal antibody that targets Retinal-specific ATP-binding cassette transporter and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Immunocytochemistry Analysis: A representative lot detected ABCA4 in Immunocytochemistry applications (Ahn, J., et. al. (2003). J Biol Chem. 278(41):39600-8).

Western Blotting Analysis: A representative lot detected ABCA4 in Western Blotting applications (Ahn, J., et. al. (2003). J Biol Chem. 278(41):39600-8; Bungert, S., et. al. (2001). J Biol Chem. 276(26):23539-46).

Immunoprecipitation Analysis: A representative lot detected ABCA4 in Immunoprecipitation applications (Ahn, J., et. al. (2003). J Biol Chem. 278(41):39600-8; Bungert, S., et. al. (2001). J Biol Chem. 276(26):23539-46).

Research Category
Neuroscience

Qualité

Evaluated by Western Blotting in human retina tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected ABCA4 in 10 µg of human retina tissue lysate.

Description de la cible

250 kDa observed; 255.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.