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MABE972

Sigma-Aldrich

Anti-PRDM5 Antibody

clone 57-20, from mouse

Synonyme(s) :

PRDM5, PR domain zinc finger protein 5, PR domain-containing protein 5

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.43

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

57-20, monoclonal

Espèces réactives

mouse

Technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG2bκ

Numéro d'accès NCBI

NP_061169

Numéro d'accès UniProt

Q9NQX1

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

mouse ... Prdm5(70779)

Description générale

PR domain zinc finger protein 5 (UniProt Q9NQX1) is encoded by the PRDM1 gene (also known as BCS2, PFM2) in human (Gene ID 11107). PRDM proteins constitute a family of transcriptional regulators that are characterized by the presence of variable numbers of zinc finger domains and an N-terminal PR domain. PRDM5 can negatively regulate gene transcriptions by recruiting the G9a histone methyltransferase and histone deacetylases. PRDM5 can promote transcription by targeting enhancer-like elements or recruiting RNA polymerase II binding. PRDM5 is highly expressed in murine embryonic stem cells (mESCs) and associates with complexes involved in chromatin organization, such as Ctcf, Smc1 (cohesin), and TFIIIC, which indicates their involvement in chromatin organization. PRDM5 is often found silenced in human breast, ovarian, liver, and cervical cancers.

Spécificité

Expected to react with all four spliced isoforms

Immunogène

GST-tagged recombinant protein corresponding to human PRDM5 near the N-terminus.

Application

Immunoprecipitation Analysis: A representative lot immunoprecipitated PRDM5 in mouse embryonic fibroblasts (Data courtesy of Dr. Anders H. Lund, University of Copenhagen, Denmark).
Immunoprecipitation: A representative lot of this antibody immunoprecipitated PRDMS E9 and E10 intrahepatic cholangiocarcinoma (ICC) cells. (Seehawer, M., et al. (2018). Nature 562(7725); 69-75).
This Anti-PRDM5 Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of PRDM5 .

Qualité

Evaluated by Western Blotting in mouse ESC lysate.

Western Blotting Analysis: 8.0 µg/mL of this antibody detected PRDM5 in 10 µg of mouse ESC lysate.

Description de la cible

~73 kDa observed. 73.1 kDa (isoform 1, calculated), 69.9 kDa (isoform 2, calculated), 12.9 kDa (isoform 3, calculated), 58.1 kDa (isoform 4, calculated).

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to lot specific datasheet.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Marco Seehawer et al.
Nature, 562(7725), 69-75 (2018-09-14)
Primary liver cancer represents a major health problem. It comprises hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC), which differ markedly with regards to their morphology, metastatic potential and responses to therapy. However, the regulatory molecules and tissue context that commit
Giorgio Giacomo Galli et al.
Molecular and cellular biology, 33(22), 4504-4516 (2013-09-18)
PRDM proteins belong to the SET domain protein family, which is involved in the regulation of gene expression. Although few PRDM members possess histone methyltransferase activity, the molecular mechanisms by which the other members exert transcriptional regulation remain to be

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