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Key Documents

GF446

Sigma-Aldrich

Thermostable bFGF,Human

HumanKine®, recombinant, Xeno-free, >95% (SDS-PAGE), suitable for cell culture

Synonyme(s) :

FGF2, prostatropin, Prostatropin, HBGF-2, BFGF

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About This Item

Code UNSPSC :
12352202
eCl@ss :
32160405
Nomenclature NACRES :
NA.75

product name

HumanKine® Thermostable bFGF,
Human Recombinant, Engineered bFGF growth factor with enchanced thermostability compared to the wild-type bFGF protein. Allows a less vigorous feeding schedule for human es/ips and neural stem cell culture.

Niveau de qualité

100

Pureté

>95% (SDS-PAGE)

Fabricant/nom de marque

HumanKine®

Impuretés

<0.5 μg/mg protein endotoxin (<5 EU/μg)

Numéro d'accès UniProt

P09038

Description générale

Product Source: HumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free.
The HumanKine Thermostable bFGF is engineered with enchanced thermostability compared to the wild-type bFGF protein without modification of biological function.

Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 154 amino acid residues. bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions.

Application

Engineered bFGF growth factor with enchanced thermostability compared to the wild-type bFGF protein. Allows a less vigorous feeding schedule for human es/ips and neural stem cell culture.
HumanKine products are Xeno-Free
Research Category
Stem Cell Research
Research Sub Category
Growth Factors & Receptors

Qualité

Bioactivity assay: Determined by the dose-dependent stimulation of the proliferation of the 3T3 cell line. EC50 is typically ≤0.5 ng/mL.

Forme physique

Lyophilized from a 0.2 μm filtered solution of 1X PBS.

Stockage et stabilité

Store at -20°C for up to 4 months from date of receipt. Further dilutions should be made into appropriate buffer or medium to which protein has been added.

Informations légales

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

HumanKine® Thermostable bFGF, an engineered recombinant protein with enhanced biological functionality on human iPS and neural progenitor cells

Fibroblast growth factors (FGFs) are secreted glycoproteins that regulate several fundamental developmental pathways and help regulate mesoderm and ectoderm patterning in the early embryonic development.

Single Transfection of a Synthetic Polycistronic Self-replicative RNA Yields High Numbers of Transgene-free Human iPSCs

The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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