ABT180

Anti-CDCP1 Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : CUB domain-containing protein 1, Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Transmembrane and associated with src kinases, CD318
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 300
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Numéro d'accès NCBI: NP_073753
• Numéro d'accès UniProt: Q9H5V8
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CDCP1(64866)

Description

Description générale

CUB domain-containing protein 1 (CDCP1) is also called Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135 kDa protein (SIMA135), Transmembrane and associated with src kinases and CD_antigen 318. CDCP1 is part of the tetraspanin web involved in tumor progression and metastasis. CDCP1 may also be involved in cell adhesion, cell matrix association and regulation of anchorage versus migration or proliferation versus differentiation via phosphorylation status. CDCP1 is a possible marker for leukemia diagnosis, and for subsets of immature hematopoietic stem cell. CDCP1 is highly expressed in mitotic cells, skeletal muscle, colon, many human tumor cell lines, colorectal cancer, breast carcinoma and lung cancer. CDCP1 may also be found in cells phenotypically similar to mesenchymal stem cells and neural stem cells.

Spécificité

Other Homologies: Mouse and Rat (77% sequence homology).

This antibody recognizes the cytoplasmic domain of CDCP1.

Immunogène

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human CDCP1.

Application

Anti-CDCP1 Antibody is a highly specific rabbit polyclonal antibody, that targets CUB domain-containing protein 1 & has been tested in western blotting & IHC (Paraffin).

Western Blotting Analysis: 2 µg/mL of this antibody detected CDCP1 in a panel of human tumor tissue lysates.

Immunohistochemistry Analysis: A 1:100 and a 1:500 dilution from a representative lot detected CDCP1 in human breast carcinoma sections.

Qualité

Evaluated by Western Blotting in human pancreas tumor tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected CDCP1 in 10 µg of human pancreas tumor tissue lysate.

Description de la cible

~135 kDa and ~70 kDa observed. Uniprot describes 3 isoforms at 92 kDa, 72 kDa, and 38 kDa, due to alternative splicing. The full length and cleaved form of CDCP1 have been observed at ~135 kDa and ~70 kDa (Casar, B., et al. (2012). Oncogene 31(35):3924-3938.). Uncharacterized band(s) may be observed in some cell lysates.

Remarque sur l'analyse

Control
Human pancreas tumor tissue lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable