ABN1672
Anti-HD-polyAla Ct
serum, from rabbit
Synonyme(s) :
HD-RAN-PolyAla, HD-PolyAla
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Forme d'anticorps
serum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
mouse, human
Conditionnement
antibody small pack of 25 μL
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
Isotype
IgG
Modification post-traductionnelle de la cible
unmodified
Description générale
Huntington disease (HD) is a progressive neurodegenerative disorder is caused by a CAG,CTG expansion in the HTT gene that results in the production of a mutant huntingtin protein (HTT) with polymeric expansions that accumulate in human brain with HD. PolyAla is a sense repeat-associated non-ATG (RAN) translation protein that accumulates in various brain regions with some toxic effects leading to microglial activation and neuronal loss. HD-RAN polyAla toxicity is shown to be lower compared to that of polyGln. The transfection of cells with polyAla construct can express high polyAla levels, but have lower toxicity. HD-polyAla RAN proteins are reported to accumulate in cells expressing CAG repeats that are equal to or greater than 52 but not at repeats less than 45. HD-RAN proteins are abundant in regions of the brain that are most affected by HD and these regions display pathologic features of HD, such as caspase-3 activity and microglial activation. HD-Ran protein accumulation and aggregation in HD brains are shown to be length dependent.
Spécificité
This rabbit polyclonal antibody specifically detects RAN proteins with PolyAla.
Immunogène
A synthetic peptides from the C-terminal regions of the predicted 21 polyAla frame of HD in the CAG direction.
Application
Anti-HD-polyAla Ct, Cat. No. ABN1672, is a highly specific rabbit polyclonal antibody that targets HD-polyAla Ct and has been tested in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Immunohistochemistry Analysis: A representative lot detected HD-polyAla Ct in Immunohistochemistry applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).
Western Blotting Analysis: A representative lot detected HD-polyAla Ct in Western Blotting applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).
Immunocytochemistry Analysis: A representative lot detected HD-polyAla Ct in Immunocytochemistry applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected HD-polyAla Ct in pcDNA3.1 (Ctrl) & HEK293T-HD-polyAla-Ct.
Western Blotting Analysis: A representative lot detected HD-polyAla Ct in Western Blotting applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).
Immunocytochemistry Analysis: A representative lot detected HD-polyAla Ct in Immunocytochemistry applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected HD-polyAla Ct in pcDNA3.1 (Ctrl) & HEK293T-HD-polyAla-Ct.
Research Category
Neuroscience
Neuroscience
Qualité
Evaluated by Western Blotting in HEK293T cells transfected with HD-polyAla-Ct.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected PolyAla in HEK293T cells transfected with HD-polyAla-Ct.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected PolyAla in HEK293T cells transfected with HD-polyAla-Ct.
Description de la cible
~15 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Forme physique
Rabbit polyclonal antiserum with 0.05% sodium azide.
Unpurified
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Monica Bañez-Coronel et al.
Neuron, 88(4), 667-677 (2015-11-22)
Huntington disease (HD) is caused by a CAG ⋅ CTG expansion in the huntingtin (HTT) gene. While most research has focused on the HTT polyGln-expansion protein, we demonstrate that four additional, novel, homopolymeric expansion proteins (polyAla, polySer, polyLeu, and polyCys)
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