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Key Documents

AB1528

Sigma-Aldrich

Anti-Nerve Growth Factor-β Antibody

serum, Chemicon®

Synonyme(s) :

NGFbeta

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

sheep

Niveau de qualité

Forme d'anticorps

serum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

rat, human, chicken, mouse

Ne doit pas réagir avec

bovine

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... NGF(4803)

Spécificité

Nerve growth factor-beta (NGF). Less than 1% cross-reactivity against recombinant human NT4 and BDNF and approximately 2% for NT3 by ELISA.

Immunogène

Mouse nerve growth factor-beta purified from salivary glands.

Application

Detect Nerve Growth Factor-β using this Anti-Nerve Growth Factor-β Antibody validated for use in ELISA, WB, IH.
Immunohistochemistry at 1:500-1:5,000 (see suggested protocol).

Immunoblotting at 1:500-1:5,000.

ELISA at 1:500-1:5,000.

Inhibition of biological activity in vitro at 1:10-1:50.
BIOLOGICAL ACTIVITY: Neutralizes NGF, but not other neurotrophins.

Use neat for in vivo animal studies (5-10 μL/g body weight).

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins

Forme physique

Whole sheep antisera. Lyophilized. Reconstitute with 250 μL of sterile distilled water.

Stockage et stabilité

Maintain at -20°C to -70°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Hiroyuki Murota et al.
The Journal of allergy and clinical immunology, 130(3), 671-682 (2012-07-10)
Itch impairs the quality of life for many patients with dermatoses, especially atopic dermatitis (AD), and is frequently induced by a warm environment. To determine the mechanism underlying itch induction by warmth, we focused on artemin, a member of glial
D Joelson et al.
Microscopy research and technique, 41(3), 246-262 (1998-05-30)
The distribution of immunoreactivity for the ionotropic N-methyl-D-aspartate (NMDA) receptor subunits was mapped in the cochlear nucleus of postnatal day (P) 7, P14, P21, and P28 gerbils. Frozen sections and serial plastic sections of tissue were incubated with antibodies to
Daniel Aridgides et al.
PloS one, 8(2), e57450-e57450 (2013-02-26)
Cardiomyocytes express neurotrophin receptor TrkA that promotes survival following nerve growth factor (NGF) ligation. Whether TrkA also resides in cardiac fibroblasts (CFs) and underlies cardioprotection is unknown. To test whether CFs express TrkA that conveys paracrine signals to neighbor cardiomyocytes
X F Zhou et al.
Journal of neuroscience methods, 54(1), 95-102 (1994-09-01)
Nerve growth factor (NGF) is a survival factor required by a number of neuronal populations including most post-ganglionic sympathetic neurones. NGF has been detected and quantified in many tissues but there is little information regarding its cellular localization. Although it
I K Abrahamson et al.
Journal of neurocytology, 16(3), 417-422 (1987-06-01)
Immunohistochemistry has been used to demonstrate the presence of nerve growth factor (NGF)-like immunoreactivity in normal and sectioned mouse sciatic nerves. In normal nerves, immunoreactive material was not visible unless a silk ligature had previously been applied to constrict the

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